ABSTRACT
Here we report our two applications of ultra-weak photon emission (UPE) measurements in agriculture. One is to find new types of agrochemicals that potentiate plants' defense, so-called "plant activator". We first analyzed the relation between plant defense and Elicitor-Responsive Photon Emission (ERPE) using a combination of rice cells and a chitin elicitor. Pharmacological analyses clarified that ERPE was generated as a part of the chitin elicitor-responsive defense in close relation with the generation of reactive oxygen species (ROS). Then we successfully detected the activity of plant activators as the potentiation of ERPE, and developed a new screening system for plant activators based on this principle. Another UPE application is to distinguish herbicide-resistant weeds from susceptible ones by measuring UPE in weeds. In our study, it was revealed that the weed biotypes resistant to sulfonylurea (SU) herbicides, one of the major herbicide groups, showed stronger UPE than susceptible ones after an SU herbicide treatment. By further analysis with a pharmacological and RNAi study, we found that the detoxifying enzyme P450s contributed to the UPE increase in SU herbicide resistant weeds. It is considered that weeds resistant to herbicides other than SU might also be able to be distinguished from susceptible ones by UPE measurement, as long as the herbicides are subject to detoxification by P450s.
Subject(s)
Agriculture , Photons , Agrochemicals/pharmacology , Cell Proliferation/drug effects , Chitin/pharmacology , Cytochrome P-450 Enzyme System/metabolism , Herbicide Resistance , Oryza/cytology , Oryza/drug effects , Oryza/metabolism , Plant Weeds/enzymology , Plant Weeds/metabolism , Sulfonylurea CompoundsABSTRACT
Following perception of herbivory or infection, plants exhibit a wide range of inducible responses. In this study, we found ultraweak photon emissions from maize leaves damaged by Helicoverpa armigera (Noctuidae). Interestingly, mechanically damaged maize leaves treated with caterpillar regurgitants emitted the same intensity and pattern of photon emissions as those from maize leaves damaged by caterpillars. Furthermore, two-dimensional imaging of the leaf section treated with the oral secretions clearly shows that photon emissions were observed specifically at the lip of the wound exposed to the secretions. These results suggest that the direct interaction between maize leaf cells and chemicals contained in caterpillar regurgitants triggers these photon emissions.
Subject(s)
Lepidoptera/pathogenicity , Photons , Zea mays/parasitology , Animal Feed , Animals , Plant Diseases/parasitology , Plant Leaves/parasitologyABSTRACT
Two strains of trichloroethylene (TCE)-degrading bacteria were isolated from soils at polluted and unpolluted sites. The isolates, strains TE26(T) and K6, showed co-substrate-independent TCE-degrading activity. TCE degradation was accelerated by preincubation with tetrachloroethylene, cis-dichloroethylene (DCE) and 1,1-DCE. TCE-degrading activities of strains TE26(T) and K6 were 0.23, 0.24 micromol min(-1) g(-1) dry cells, respectively. 16S rDNA sequences of strains TE26(T) and K6 were almost identical (99.7% similarity), and most closely related to Ralstonia basilensis (ATCC17697(T)) (98.5% similarity). From the results of DNA-DNA hybridizations, strain TE26(T) was genetically coherent to strain K6 (94 and 88% hybridization), and exhibited lower relatedness to R. basilensis (DSM11853(T)) (44% and 15%). In addition, because of the differences in chemotaxonomic properties, strain TE26(T) and strain K6 appear to be distinct from all established species of the Ralstonia group. Based on these results and the proposal of transferring R. basilensis and related species to Wautersia gen. nov., we propose that these strains should be assigned to the genus Wautersia as Wautersia numadzuensis sp. nov.
