ABSTRACT
A simple, precise, rapid and selective HPLC-RP method has been developed for the simultaneous determination of thiamine hydrochloride (B(1)) 150 mg, pyridoxine hydrochloride (B(6)) 150 mg, and hydroxocobalamine chloride (B(12)) 0.150 mg in multivitamin tablets. The method uses a Hypersil-BDS C(18) reversed phase column and gradient elution. The aqueous mobile phase contained 0.015% triethylamine adjusted to pH 2.7 with 1 N sulfuric acid and acetonitrile. Separation and quantitation was achieved by changing the proportion of the system linearly with a time-schedule programme. Detection was carried out using a dual-beam UV detector set at 280, 350 nm. Good linearity was observed between the concentration of the analytes and peak area (r=0.9999, 0.9998). Sample preparation was relatively simple whereas excipients present in the dosage forms did not interfere with the peaks of interest. Recovery of the compound from the B(1), B(6), B(12) was quantitative.
Subject(s)
Thiamine/analysis , Vitamin B 12/analysis , Vitamin B 6/analysis , Chemistry, Pharmaceutical , Chromatography, High Pressure Liquid/methods , TabletsABSTRACT
In this study a complex of Ibuprofen and b-Hydroxypropylcyclodextrin was prepared employing a freeze drying method. The production parameters and the final specifications of this product were optimized by using response surface methodology. The results show that the freeze dried complex meets the requirements for solubility to be considered as a possible injectable form.
