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1.
Phys Chem Chem Phys ; 17(11): 7507-13, 2015 Mar 21.
Article in English | MEDLINE | ID: mdl-25706609

ABSTRACT

Not only drastic temperature- but also pressure-induced perturbations of membrane organization pose a serious challenge to the biological cell. Although high hydrostatic pressure significantly influences the structural properties and thus functional characteristics of cells, this has not prevented life from invading the high pressure habitats of marine depths where pressures up to the 100 MPa level are encountered. Here, the temperature- and pressure-dependent structure and phase behavior of giant plasma membrane vesicles have been explored in the absence and presence of membrane proteins using a combined spectroscopic and microscopic approach. Demixing into extended liquid-ordered and liquid-disordered domains is observed over a wide range of temperatures and pressures. Only at pressures beyond 200 MPa a physiologically unfavorable all gel-like ordered lipid phase is reached at ambient temperature. This is in fact the pressure range where the membrane-protein function has generally been observed to cease, thereby shedding new light on the possible origin of this observation.


Subject(s)
Cell Membrane/chemistry , Pressure , Temperature , Animals , Cell Line, Tumor , Membrane Proteins/chemistry , Rats
2.
Methods ; 76: 67-77, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25168090

ABSTRACT

Pressure perturbation calorimetry (PPC) is an efficient technique to study the volumetric properties of biomolecules in solution. In PPC, the coefficient of thermal expansion of the partial volume of the biomolecule is deduced from the heat consumed or produced after small isothermal pressure-jumps. The expansion coefficient strongly depends on the interaction of the biomolecule with the solvent or cosolvent as well as on its packing and internal dynamic properties. This technique, complemented with molecular acoustics and densimetry, provides valuable insights into the basic thermodynamic properties of solvation and volume effects accompanying interactions, reactions and phase transitions of biomolecular systems. After outlining the principles of the technique, we present representative examples on protein folding, including effects of cosolvents and crowding, together with a discussion of the interpretation, and further applications.


Subject(s)
Calorimetry/methods , Protein Folding , Proteins/chemistry , Muramidase/chemistry , Polystyrenes/chemistry , Ribonuclease, Pancreatic/chemistry , Solvents/chemistry , Temperature , Thermodynamics , Water/chemistry
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