Value of urinary liver fatty acid-binding protein in assessing severity of brain trauma and predicting acute kidney injury / 南方医科大学学报

<p><b>OBJECTIVE</b>To assess the value of urinary liver-type fatty acid-binding protein (L-FABP) in early assessment of the severity of traumatic brain injury and in predicting the occurrence of acute kidney injury (AKI) following the brain injury.</p><p><b>METHODS</b>Sixty-five patients with traumatic brain injury patients were divided into 4 groups according to their Glasgow coma scale (GCS) scores. Blood and urine samples were collected at 2, 6, 12, 24, 48 and 72 h after the injury to detect serum creatinine (SCr) level using biochemical analyzer and urinary L-FABP using enzyme-linked immunosorbent assay (ELISA), with samples from 15 healthy adults as controls. The correlations were analyzed among SCr, urinary L-FABP, GCS score upon admission and AKI occurrence.</p><p><b>RESULTS</b>The patients with moderate to severe brain injuries showed significantly higher SCr and urinary L-FABP levels than the control group (P<0.05). GCS score of the patients was inversely correlated with the levels of SCr and urinary L-FABP (P<0.05), and the changes were more prominent in urinary L-FABP than in SCr. The incidence of AKI was 21.54% in these patients. In patients with AKI, urinary L-FABP reached the peak level as soon as 6 h after the injury, as compared with 24 to 48 h when peak SCr level occurred.</p><p><b>CONCLUSION</b>Urinary L-FABP can be used as a marker for early assessment of the severity of traumatic brain injury and for predicting the occurrence of AKI following the injury.</p>

Single nucleotide polymorphisms of the DGKK gene and hypospadias in Chinese children / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the role of single nucleotide polymorphisms of the gene of diacylglycerol kinase κ (DGKK) in hypospadias in Chinese children.</p><p><b>METHODS</b>We performed direct sequencing on 2 hypospadias-related candidate single nucleotide polymorphisms of the DGKK gene (rs1934179 and rs7063116, never previously reported in the Chinese population) from 300 children with sporadic hypospadias and 200 healthy controls, and compared the results between the two groups.</p><p><b>RESULTS</b>The mutation frequencies of rs1934179 and rs7063116 were 5.0% (15/300) and 5.67% (17/300) respectively in the hypospadias patients, significantly higher than 1.5% (3/200) and 2.0% (4/200) in the normal controls (P <0.05). The mutation frequencies of rs1934179 and rs7063116 in the cases of distal and middle hypospadias were also remarkably higher (6.5%, [13/200] and 7.5% [15/200], P <0.05), but those in the proximal cases (both 2.0% [2/100]) showed no statistically significant difference from the control (P >0.05).</p><p><b>CONCLUSION</b>The polymorphisms of the DGKK gene may be associated with hypospadias, particularly distal and middle hypospadias, in Chinese children.</p>

Single-nucleotide polymorphisms of MAMLD1 and hypospadias in Chinese / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the role of the MAMLD1 gene mutation in the pathogenesis of hypospadias in the Chinese population.</p><p><b>METHODS</b>We collected peripheral venous blood from 150 Chinese children with hypospadias (the case group) and another 120 normal healthy ones (the control group), aged 0.5 to 6 years. We obtained their DNA samples and performed DNA sequencing on the single-nucleotide polymorphisms of MAMLD1, followed by comparative analysis.</p><p><b>RESULTS</b>A known missense mutation polymorphism p. N589S was identified in 12 (8.0%) of the hypospadias patients and 4 (3.0%) of the normal controls, and a novel missense mutation polymorphism p. N567S was identified in 4 (2.7%) of the patients and 3 (2.5%) of the controls, neither with statistically significant differences between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>The results re-emphasized the importance of replication in genetic association approaches, and might reveal a real difference in susceptibility genes among different populations. The single-nucleotide polymorphisms of MAMLD1 bear no obvious correlation with hypospadias, and MAMLD1 is not a candidate gene in its pathogenesis in the Chinese population.</p>

Synthesis of biflavones and their interaction with DNA / 药学学报

To explore new biflavones, 7-hydroxy-8-hydroxymethyl-4'-methoxyisoflavone (1), (5, 7-dihydroxyflavone-8-yl)-(7'-hydroxy-4"-methoxyisoflavone-8'-yl)methane (2), bis(7-hydroxy-4'-methoxyflavone-8-yl) methane (3), bis(3', 5'-diisopropyl-7, 4'-dihydroxy-isoflavone-8-yl)methane (4), and bis(7-hydroxy-isoflavone-8-yl) methane (5) were designed and synthesized from chrysin, formononetin, 7, 4'-dihydroxy-3', 5'-diisopropyl-isoflavone and 7-hydroxy-isoflavone. Their structures were identified with IR, 1H NMR, 13C NMR and elemental analysis. The binding of 1-5 with DNA was studied with fluorescent spectroscopy. Compounds 2-5 showed higher binding affinity with DNA than 1. According to the Stern-Volmer equation, the binding constants of 2, 3 were determined at 35 degrees C and 25 degrees C respectively, they were Kq2 (25 degrees C) = 1.95 x 10(4) Lx mol(-1) and Kq2 (35 degrees C) = 1.67 x 10(4) L x mol(-1); Kq3 (25 degrees C) = 1.89 x 10(4) L x mol(-1) and Kq3 (35 degrees C) = 1.58 x 10(4) L x mol(-1). The quenching mechanism of 2, 3 was suggested as static quenching.

Clinical application of microsurgical shunt in treatment or varicocele with oligospermia / 中华显微外科杂志

Objective To study the therapeutic efficacy and the application value of microsurgical shunt in varicocele (VC) with oligospermia. Methods Funicular vein elevatus ligation and microsurgical shunt were applied in 66 patients. Improvements of the symptom, sign,semen quality and the couple's pregnant rate in prognosis visiting were observed. Results in 71 sides by microsurgical shunt,symptom disappeared in 51 cases and obviously improved in 20 cases after 7 days. Half and one year later,symptom and sign were disappeared in 69 cases (97% );semen quality was improved in 52 cases (78% ). In 37 cases (56. 3% ),sperm density was ≥20 ? 109/L, progress sperm was ≥50% and morphologically normal sperm was ≥30 %. In 11 cases (16. 7% ),sperm density was increased from 5 ? 10,/L- 15 ? 109/L before operation to 10 ? 109/L- 19 ? 109/L after operation,and both of the sperm motility and viability were increased. In 4 cases (5% ),sperm density was increased from less than 5 ? 10s/L before operation to more than 5 ? 10,/L after operation. In 26 cases,the couple was pregnant;the pregnant rate was 39. 4%' Conclusion Vein elevatus ligation and microsurgical shunt blocked the vein blood reflux,established the new vein circuit and improved the blood supply of testis in the mean time. It was approved that the method was effective in diminishing the local symptom and sign, improving semen quality and preventing recurrence' It was especially suitable for classⅢ exudative VC patients