ABSTRACT
We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.
Subject(s)
Cholesterol/analogs & derivatives , Luciferases/genetics , Transfection/methods , Animals , Blood , Cell Line , Cholesterol/chemical synthesis , Culture Media , Drug Carriers , Endothelium, Vascular , Genes, Reporter , Humans , Liposomes , Melanoma, Experimental , Mice , Phosphatidylethanolamines , Plasmids , Tumor Cells, CulturedABSTRACT
Studies on the pulmonary respiration function in lung cancer patients by roentgenopneumopolygraphy allowed determining some reduction in the level of the lung parenchyma ventilatory activity on average up to 0.20 +/- 0.02 unit of optic density. In peripheral lung cancer the reduction in lung functioning was on average up to 0.30 UOD. As a whole, this index in lung cancer patients compared with normal was reduced by 0.40 UOD. Moreover, increasing of the lung surface area was noted during inspiration and expiration with the corresponding decrease of pulmonary distension. A coefficient of respiratory chest expansion reached on average 82 +/- 2.54%. Roentgenopneumopolygrams showed distinctly the symptom of mediastinal organs displacement to the site of pathology, thus indicating bronchostenosis. Also, a leveling of optic density of expiratory and inspiratory bands on the side of affection was, noted. Thus, roentgenopneumopolygraphy as a functional method of investigation makes it possible to reveal precisely enough the symptoms of partial bronchial obturation, atelectasis, distension of the lung as well as to determine the degree of gravity of the lesion concerned.
