ABSTRACT
The qualitative and quantitative changes in the bacterial community composition in two mesophilic, commercially used biogas plants were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. The main objective was to evaluate the influence of the co-substrate maize silage on total bacteria and some selected bacterial groups by comparing full-scale reactors fed solely with pig manure or additionally with maize silage. DGGE fingerprints reflected shifts in the bacterial community structure associated with maize silage as co-substrate and the real-time PCR results showed clear changes in the quantitative composition of the bacterial consortia of each fermenter. A clear dominance of Clostridia in all surveyed fermenters and considerably lower abundance of Bacteroidetes in the biogas plant fed with maize silage was shown.
Subject(s)
Bacteria/isolation & purification , Bacteria/metabolism , Biofuels/microbiology , Manure/microbiology , Silage/microbiology , Zea mays/microbiology , Animals , Bacteria/classification , Bacteria/genetics , Biodiversity , Biofuels/analysis , Bioreactors/microbiology , Culture Media/analysis , Culture Media/metabolism , Manure/analysis , Molecular Sequence Data , Phylogeny , Silage/analysis , Zea mays/chemistryABSTRACT
The findings in cardiotocograms of 18 patients with placental abruption were analysed. Eleven out of 16 cases monitored during the 2-h period preceding delivery had five or more contractions in 10 min, with a mean of 5.6. Signs of fetal distress in heart rate recordings in the same period were found in 14 monitored cases. The diagnostic value of the cardiotocogram, especially the interpretation of tocograms in placental abruption, is stressed.
