Testate amoebae taxonomy and trait diversity are coupled along an openness and wetness gradient in pine-dominated Baltic bogs.

Sphagnum peatlands host a high abundance of protists, especially testate amoebae. Here, we designed a study to investigate the functional diversity of testate amoebae in relation to wetness and forest cover in Baltic bogs. We provided new data on the influence of openness/wetness gradient on testate amoebae communities, showing significant differences in selected testate amoebae (TA) traits. Three key messages emerged from our investigations: 1) we recorded an effect of peatland surface openness on testate amoebae functional traits that led us to accept the hypothesis that TA traits differ according to light intensity and hydrology. Mixotrophic species were recorded in high relative abundance in open plots, whereas they were nearly absent in forested sites; 2) we revealed a hydrological threshold for the occurrence of mixotrophic testate amoebae that might be very important in terms of peatland functioning and carbon sink vs. source context; and 3) mixotrophic species with organic tests were nearly absent in forested sites that were dominated by heterotrophic species with agglutinated or idiosomic tests. An important message from this study is that taxonomy of TA rather indicates the hydrological gradient whereas traits of mixotrophs the openness gradient.

Unveiling tipping points in long-term ecological records from Sphagnum-dominated peatlands.

Unveiling past tipping points is a prerequisite for a better understanding of how individual species and entire ecosystems will respond to future climate change. Such knowledge is key for the implementation of biodiversity conservation. We identify the relationships between peatland vegetation and hydrological conditions over the past 2000 years using plant macrofossils, testate amoebae-based quantitative hydrological reconstructions and Sphagnum-moss functional traits from seven Polish peatland records. Using threshold indicator taxa analysis, we discovered that plant community composition strongly converged at a water level of ca 11.7 cm, indicating a community-level tipping point. We identified 45 plant taxa that showed either an increase or a decrease in their relative abundance between 8 and 17 cm of water-level depth. Our analysis of Sphagnum community traits further showed that Sphagnum functional diversity was remarkably stable over time despite Sphagnum species sensitivity to hydrological conditions. Our results suggest that past hydrological shifts did not influence major functions of the Sphagnum community, such as photosynthetic capacity, growth and productivity, owing to species replacement with a similar functional space. Although further studies including trait plasticity will be required, our findings suggest that the capacity of the Sphagnum community to gain carbon remained stable despite hydrological changes.