ABSTRACT
Drought and osmotic stresses are major threats to agricultural crops as they affect plants during their life cycle. The seeds are more susceptible to these stresses during germination and establishment of seedlings. To cope with these abiotic stresses, various seed priming techniques have broadly been used. The present study aimed to assess seed priming techniques under osmotic stress. Osmo-priming with chitosan (1 and 2%), hydro-priming with distilled water, and thermo-priming at 4 °C were used on the physiology and agronomy of Zea mays L. under polyethylene glycol (PEG-4000)-induced osmotic stress (-0.2 and -0.4 MPa). The vegetative response, osmolyte content, and antioxidant enzymes of two varieties (Pearl and Sargodha 2002 White) were studied under induced osmotic stress. The results showed that seed germination and seedling growth were inhibited under osmotic stress and germination percentage, and the seed vigor index was enhanced in both varieties of Z. mays L. with chitosan osmo-priming. Osmo-priming with chitosan and hydro-priming with distilled water modulated the level of photosynthetic pigments and proline, which were reduced under induced osmotic stress; moreover, the activities of antioxidant enzymes were improved significantly. In conclusion, osmotic stress adversely affects the growth and physiological attributes; on the contrary, seed priming ameliorated the stress tolerance resistance of Z. mays L. cultivars to PEG-induced osmotic stress by activating the natural antioxidation enzymatic system and accumulating osmolytes.
ABSTRACT
Plants are sessile and mostly exposed to various environmental stresses which hamper plant growth, development, and significantly decline its production. Drought stress is considered to be one of the most significant limiting factors for crop plants, notably in arid and semi-arid parts the world. Therefore, the present study aimed to evaluate the potential impact of different concentrations (10, 100, and 200 µg/mL) of kinetin capped zinc oxide nanoparticles (Kn-ZnONPs) on Vigna radiata (L.) R. Wilczek under varying levels (5%, 10%, 15%) of PEG-induced drought stress. ZnONPs were synthesized by a co-precipitation method using Zinc acetate as a precursor at pH-12, incinerated to 500 °C, and kinetin was used as a surface functionalizing agent. The resulting Kn-ZnONPs were characterized by various contemporary analytical techniques, including SEM, SEM-EDS, XRD, DLS, and Zeta potential and IR spectroscopy. Crystalline Kn-ZnONPs, with a zeta potential of 27.8 mV and a size of 67.78 nm, of hexagonal wurtzite structure and vibrational stretches associated with N-H, C-O, C-N, etc., were confirmed. PEG-induced drought stress significantly reduced the growth of V. radiata by declining the chlorophyll and carotenoid contents. Moreover, a significant decrease in the levels of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), soluble sugar contents, proline, protein contents, phenol, and tannin were observed compared to the control. However, the exogenous application of Kn-ZnONPs ameliorated all photosynthetic parameters by up-regulating the antioxidant defense system through the promotion of SOD, POD, CAT, and lipid peroxidation levels. The biochemical parameters, such as proteins, soluble sugars, and proline, were observed to be maximum in plants treated with 200 µg/mL Kn-ZnONPs under 5% drought stress. The application of Kn-ZnONPs also enhanced the total phenol contents, flavonoid, and tannin contents. In conclusion, the findings of this study demonstrate that the exogenous application of Kn-ZnONPs provides beneficial effects to V. radiata by attenuating the damaging effects of drought stress through the up-regulation of the antioxidant defense system and osmolytes. These results suggest that Kn-ZnONPs have potential as a novel approach to improve crop productivity under drought stress conditions.
