ABSTRACT
The antigenic properties of virus isolates of tobacco mosaic, cucumber mosaic, bean common and yellow mosaic, soybean mosaic, Y-, X-, and A-potato viruses, Vicia unijuga mosaic, affecting agricultural crops and wild plants in the Far East of Russia were studied. Universal immunodiagnosticums fit for testing the broad range of virus isolates have been obtained on the basis of the most antigen-active strains. They allow to struggle effectively against viruses rendering economic damage to agriculture.
Subject(s)
Bromovirus/isolation & purification , Cucumovirus/isolation & purification , Immunoglobulin G/biosynthesis , Potexvirus/isolation & purification , Potyvirus/isolation & purification , Reagent Kits, Diagnostic , Tobamovirus/isolation & purification , Animals , Asia, Eastern , Immunoassay , Immunoglobulin G/chemistry , Plants/virology , Rabbits , SiberiaABSTRACT
The new virus isolated from Vicia unijuga A.Br. with filament particles with size 1000-1200 x 10-12 nm is revealed. A thermal inactivation point is 55 degrees C; dilution end point - 10(-5)-10(-6) longevity in vitro in broad bean sap--less than one day. It is transferred by aphids and by pea, bean and broad bean seeds. The plants of Fabaceae, Solanaceae and Chenopodiaceae fam. were affected by this virus isolate. The virus yield was 40-50 mg per 100 g of leaves. The ratio of absorption E260/E280 corresponded to 1.4-1.5. The molecular mass of a core protein of the virus was 34 kD. The virus has a high immunogenic properties--titer is 1:256000 (indirect method of ELISA). It is presumably identified as a member of Closteroviridae.
Subject(s)
Closteroviridae/isolation & purification , Plant Diseases/virology , Vicia/virology , Capsid Proteins/chemistry , Closteroviridae/chemistry , Plant Leaves/virology , Virion/chemistry , Virion/isolation & purificationABSTRACT
The distribution and accumulation of soybean mosaic virus antigen in the leaves of sensitive plants with well expressed mosaic symptoms and symptomless tolerant soybean plants Glycine max (L.) Merr. were similar. Low concentration of virus antigen was found in extremely resistant soybean plants in mechanically inoculated leaves and those of the 2-4 circles two-three weeks after inoculation but 5 weeks later the virus antigen was detected only in the top leaves.
