Sushi-roll wrap of Seprafilm for ileostomy limbs facilitates ileostomy closure.

BACKGROUND: Sodium hyaluronate and carboxymethylcellulose membrane (Seprafilm) reduced postoperative intraabdominal adhesion. In this study, we examined whether Seprafilm reduces operative difficulties in ileostomy closure. PATIENTS AND METHODS: During the creation of the ileostomy, Seprafilm was cut in half and used to wrap both the ileum and mesentery. Patients who underwent ileostomy closure before February 2008 (without Seprafilm, Group T, n = 18) and after March (with Seprafilm, Group S, n = 18) were enrolled in this study. All operations were performed by surgical residents. Operative time and perioperative complications were analyzed. RESULTS: The mean operative time of Group S (106.88 min) was significantly less than that of Group T (120.6 min). The amount of intraoperative bleeding in Groups S and T was not significantly different and there were no major complications. CONCLUSION: Seprafilm applied to the two limbs of the ileostomy and mesentery facilitate ileostomy closure done by non-expert surgeons.

Studies on myocardial mitochondria in ischemic dog hearts by electron spin resonance (ESR) spectrometry.

The free radical concentration of myocardial mitochondria from ischemic dog heart was studied by means of electron spin resonance (ESR) spectrometry, and the effects of acute free fatty acid (FFA) mobilization on the process of electron transport and oxidative phosphorylation of ischemic dog heart mitochondria were studied using free radical concentration in the state IV respiration of the myocardial mitochondria as the indicators. 1. The intensity of the ESR signal of mitochondria (g = 2.003) from intact dog heart was significantly increased in state IV compared to state I (state IV/state I = 2.01 +/- 0.18). On the other hand, in state III, the ESR intensity was markedly decreased compared to state IV (state III/state IV = 0.33 +/- 0.06). 2. The ESR intensity ratio of state IV/state I of the mitochondria from the ischemic area of 1-hr ischemic dog heart decreased remarkably compared to normal group. 3. The ESR intensity ratio of state IV/state I of the mitochondria isolated from the ischemic area of 2-hr ischemic dog heart was significantly lower than in the control. The ESR intensity ratio of state IV/state III of this group was significantly higher than in the control. 4. In ischemic heart with high plasma FFA, the free radical concentration in state IV respiration of the myocardial mitochondria was significantly decreased as compared to ischemic heart without high plasma FFA. These results suggest that the free radical concentration of myocardial mitochondria in respective respiratory states may be useful for evaluation of functional disturbance of oxidative phosphorylation and the electron transfer system in myocardial mitochondria in ischemic heart. Sudden and abnormal rise of plasma FFA seems to further facilitate the uncoupling of oxidative phosphorylation in the mitochondria of the ischemic portion of the heart.

Studies on myocardial mitochondria in failing dog hearts: studies by electron spin resonance (ESR) spectrometry.

The formation of free radicals, which was represented by the concentration of free radicals in state 4 respiration, and the respiratory control ratio were studied in the myocardial mitochondria isolated from dog hearts that were failing due to aortic constriction. Both formation and respiratory control ratio were lowered, compared to each control value, in acute and chronic heart failure. A positive correlation was recognized between free radicals in state 4 respiration and respiratory control ratio. From these results it was concluded that there was uncoupling of oxidative phosphorylation in the mitochondria isolated from failing dog hearts. Measurement of free radicals in state 4 respiration, as well as respiratory control ratio, is a useful method for evaluation of the function of oxidative phosphorylation.

Arrhythmogenic effects of acute free fatty acid mobilization on ischemic heart.

The mechanism of action of the acute abnormal rise of plasma free fatty acids (FFA) in the provocation of arrhythmia in ischemic heart was studied by means of electron spin resonance (ESR) spectrometer. A sudden and abnormal rise of plasma FFA caused a significant fall of the respiratory control index (RCI) of the amount of free radical myocardial mitochondria in state 4 respiration. Based on these findings, a sudden and abnormal rise of plasma FFA seems to further facilitate the uncoupling of oxidative phosphorylation in the myocardial mitochondria of the ischemic portion of the heart. These observations indicate that it may play an important role in the provocation of arrhythmia by high plasma FFA on the ischemic heart. Nicomol (2,2,6,6,-tetrakis (nicotinoyloxymethyl) cyclohexanol), an inhibitor for the rapid rise of plasma FFA, was effective in the treatment and prevention of arrhythmia in ischemic heart disease and diabetes mellitus.

Dopamine-beta-hydroxylase activity after acute myocardial infarction.

After acute myocardial infarction, serial serum dopamine-beta-hydroxylase (DBH) activity was elevated in both high and low DBH subgroups. The observed increase in DBH activity on the first, second, and third days after acute myocardial infarction suggests an augmentation in sympathetic nervous system activity after acute myocardial infarction.