ABSTRACT
For optimizing CD34+ cell collection, appropriately timing peripheral blood stem cell harvest (PBSCH) initiation is crucial. Automatic cell analyzers with the immature myeloid information channel provide hematopoietic progenitor cell (HPC) count, a surrogate marker of CD34+ cells, which can be obtained within a few minutes without requiring monoclonal antibodies. The final decision on PBSCH initiation can be made using the HPC count obtained on the morning of the harvest day. Herein, we evaluated the impact of the HPC count as an indicator for the optimal timing of PBSCH in clinical practice over 9 years. One hundred and eighteen aphereses from 72 cases had a definite number of CD34+ cells/kg in the PBSC yield. A correlation was found between the HPC count in the PB and the CD34+ cell count (R = 0.563, p ï¼ 0.001), whereas no correlation existed between the white blood cell and CD34+ cell counts (R = 0.0418, p = 0.65). We defined ï¼ 2.0 × 106/kg of CD34+ cells in a single apheresis as good mobilization. Multivariate analysis demonstrated that an HPC count of ï¼ 21/µL, myeloblast count of ï¼ 12/µL, and age at PBSCH of ï¼ 50 years were independently associated with good mobilization (p = 0.001, p ï¼ 0.001, and p = 0.005, respectively). Our findings suggest that the HPC count is a good indicator for the optimal timing of PBSCH.
Subject(s)
Antigens, CD34/analysis , Hematopoietic Stem Cell Mobilization/methods , Hematopoietic Stem Cells/cytology , Peripheral Blood Stem Cells/cytology , Adult , Female , Hematopoietic Stem Cell Mobilization/standards , Humans , Leukapheresis/methods , Leukapheresis/standards , Leukocyte Count , Male , Middle Aged , Peripheral Blood Stem Cell Transplantation/methods , Practice Patterns, Physicians'/standards , Time FactorsABSTRACT
A method was developed for the determination of (129)I in soil samples that uses an ICP-MS equipped with an octopole reaction system. Oxygen was used as the reaction gas for reducing the background intensity of m/z 129, principally by (129)Xe(+). The contribution of polyatomic ions, such as (127)IH(2)(+), could be effectively corrected for by assuming a production ratio ((127)IH(2)(+)/(127)I(+) = 3 × 10(-8)). The measured (129)I/(127)I ratios in a NIST standard solution and Fukushima soil samples are consistent with the expected value within the analytical error. This method provides a powerful tool for investigating radioiodine contamination in Fukushima and elsewhere.
