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Ukr Biokhim Zh (1978) ; 64(6): 38-42, 1992.
Article in Ukrainian | MEDLINE | ID: mdl-1488811

ABSTRACT

Interaction of the bovine liver tRNA(GCUSer) having a long variable loop, with the cognate aminoacyl-tRNA synthetase has been studied by alkylation with ethylnitrosourea. It was shown that seryl-tRNA synthetase protects 3'-phosphates of nucleotides 12, 13 in D-stem and 45-47-, 47 G.-, 47 H-variable stem of tRNA(GCUreS) from alkylation. An anticodon loop of tRNA(GCUSer) did not interact with seryl-tRNA synthetase.


Subject(s)
Amino Acyl-tRNA Synthetases/metabolism , Liver/metabolism , RNA, Transfer, Ser/metabolism , Alkylation , Amino Acyl-tRNA Synthetases/drug effects , Animals , Binding Sites , Cattle , Drug Interactions , Ethylnitrosourea/pharmacology , In Vitro Techniques , Liver/drug effects , RNA, Transfer, Ser/drug effects , RNA, Transfer, Ser/isolation & purification
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