Design and Development of Modified Ensemble Learning with Weighted RBM Features for Enhanced Multi-disease Prediction Model.

In this computer world, huge data are generated in several fields. Statistics in the healthcare engineering provides data about many diseases and corresponding patient's information. These data help to evaluate a huge amount of data for identifying the unknown patterns in the diseases and are also utilized for predicting the disease. Hence, this work is to plan and implement a new computer-aided technique named modified Ensemble Learning with Weighted RBM Features (EL-WRBM). Data collection is an initial process, in which the data of various diseases are gathered from UCI repository and Kaggle. Then, the gathered data are pre-processed by missing data filling technique. Then, the pre-processed data are performed by deep belief network (DBN), in which the weighted features are extracted from the RBM regions. Then, the prediction is made by ensemble learning with classifiers, namely, support vector machine (SVM), recurrent neural network (RNN), and deep neural network (DNN), in which hyper-parameters are optimized by the adaptive spreading rate-based coronavirus herd immunity optimizer (ASR-CHIO). At the end, the simulation analysis reveals that the suggested model has implications to support doctor diagnoses.

Decolorization of safranin using Fissidens species and its ecotoxicological assessments: An in vitro and in silico approach.

Decolorization of safranin was investigated using Fissidens species in a batch system under optimized conditions. The decolorization efficiency was improved by optimizing the conditions such as initial pH (3-9), temperature (25-45 °C), initial dye concentration (10-50 mg/L), biosorbent dosage (100-500 mg/L) and contact time (1-6 days). Maximum decolorization (95%) was recorded at initial pH of 6 with dye concentration of 20 mg/L, biosorbent dosage of 200 mg/L at 30 °C and contact time of 2 days. Desorption studies revealed 0.1 N NaOH as the best desorbing agent with 92% recovery on third day. Experimental data well fitted to Langmuir isotherm and Pseudo-second order kinetic model. The negative values of ΔGo and positive value of ΔSo and ΔHo indicates that the reaction is spontaneous, favorable and endothermic. The biosorbent - dye interactions were confirmed using UV-Vis, FT-IR, XRD and FE-SEM with EDX studies. The detoxified nature of the dye degraded metabolites was confirmed by the significant growth of green gram. The color fastness and color strength of the fabrics dyed using Fissidens species treated dye solution were compared with the tap water dyed fabrics which indicated the reuse potential of treated water in textile sector. The decolorization efficiency was further confirmed through in silico approach, where safranin well docked with the active sites of Photosystem II protein D1 of the Fissidens species. Thus, the present study proves that Fissidens species is a promising biosorbent for safranin decolorization and will lay a platform for the control and management of environmental pollution.

Influence of plant growth regulators (PGRs) and various additives on in vitro plant propagation of Bambusa arundinacea (Retz.) Wild: A recalcitrant bamboo species.

An efficient micropropagation protocol for high frequency plant regeneration was developed using nodal explants derived in vitro seedlings of Bambusa arundinacea which is an important multipurpose and edible bamboo species and recalcitrant to tissue culture. The nodal explants excised from 20-day-old seedlings were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of 6-benzyl amino purine (BAP) and kinetin (KIN) (0.5-5.0 mg/l) alone and/or in combination with 0.5 mg/l of different auxins [indole-3-butyric acid (IBA) α-naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA)] for shoot bud induction. The combination of BAP (3.0 mg/l) and IBA (0.5 mg/l) was found to be the best for the highest percent of shoot bud initiation (87.2%), with 24.2 shoots/explant. The highest frequency (95.2%) of shoot bud multiplication with maximum number of shoots (90.5 shoots/culture) was noticed on medium containing 4% coconut water with 4% sucrose. The regenerated shoot buds were cultured on MS medium supplemented with various concentrations of auxins alone and/or in combination with AgNO3 (0.5-4.0 mg/l) for in vitro rooting. Maximum percent of rooting (85%) was noticed on MS medium augmented with 3.0 mg/l IBA and 2.0 mg/l AgNO3 after 14 days of culture. Well rooted plantlets obtained were established in the field with 92% survival rate. The present plant regeneration protocol could be used for large scale propagation and ex-situ conservation of this important bamboo species in the near future.

Decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigatus

The aim of this work was to study the decolourization of textile dye effluent by non-viable biomass of Aspergillus fumigates. The dried non-viable fungal biomass exhibited maximum dye removal at pH 7.0 with temperature of 30ºC and 3 g/l (w/v) biomass concentration, after 24 h contact time. The results showed that the non-viable biomass possessed high efficiency for dye removal from textile effluent.