ABSTRACT
Background: Teeth are one of the strongest hard tissues that can resist a variety of antemortem and postmortem insults and an important evidence in the field of medicolegal and forensics. In the identification of unknown human remains of the deceased, stature estimation is a preliminary investigation. Previously, the comparison of osteometry with odontometry in stature determination has been made. Similarly, an attempt is made to estimate stature using intercanine and intermolar width which may be a forensic tool in the future. Aim and Objective: The aim of this study was to determine stature using intercanine and intermolar width in the maxillary and mandibular arches and correlating to identify highly reliable parameter among the two. Further to evaluate the usefulness of odontometry in stature determination. Materials and Methods: Subjects for the study were recruited from the outpatient department of APDCH and students of APDCH. One hundred subjects were included in the study comprising fifty males and fifty females within the age group of 17-28 years as per inclusion criteria. Digital Vernier caliper for measuring intercanine width (cusp tips on either sides) and intermolar width (central fossa of 1st molar on either sides) on the study models and standard anthropometer for measuring the height of the subject (vertex to the floor). Results: Compared to other parameters, the maxillary intercanine distance is statistically significant to P = 0.05 with correlation value of 0.06 by Pearson correlation method. Conclusion: The data collected were subjected to statistical analysis and was found that out of the four selected odontometric parameters, maxillary intercanine width with a P = of 0.032 and Pearson correlation of 0.307 in Group 1 (males) were found to show a significant correlation with height. Regression analysis was done, and regression equations were derived. The following regression equation can be used to calculate the height of an individual, Group 1 (male) -Y = B+ Ax. Y = 135.5 + 0.947X (X - maxillary inter canine width). This method of stature prediction may be of relative importance but not of absolute certainty, thereby making it an adjunct to various other methods of stature prediction. Therefore, using odontometric parameters in combination improves the predictability of estimating stature rather than a single parameter.
ABSTRACT
Background: The study of cell proliferation is important for assessing the tumor behavior, prognosis and patient survival of oral carcinomas. As literature search did not reveal sufficient studies of immunohistochemical expression of cyclin D1 and minichromosome maintenance 2 (MCM2) in oral squamous cell carcinoma (OSCC) and verrucous carcinoma (VC), the present study was undertaken. Materials and Methods: The study group included 20 cases of histopathologically diagnosed OSCC, 10 cases of VC and 10 cases of normal mucosa (NM). All samples were evaluated for the expression of cyclin D1 and MCM2 using standard Immunohistochemistry (IHC) procedure.The present study involved both qualitative and quantitative analyses. Qualitative analysis was done by evaluation of intensity and area of staining. Quantitative analysis was done by calculating the percentage of positively stained cells and assessing the labeling index (LI). Data obtained were subjected to statistical analysis using SPSS statistical package (version 23.0). Results: On evaluating and comparing the intensity of staining and area of staining of cyclin D1 and MCM2 between the study groups, statistically significant values (P < 0.05) were obtained using Kruskal-Wallis ANOVA. Comparison of LI of cyclin D1 and MCM2 in NM, OSCC and VC statistically significant results (P < 0.05) was obtained using Mann-Whitney U-test. Mean LI of MCM2 was found to be significantly higher than mean LI of cyclin D1 in all the study groups. Conclusion: From the present study, we conclude that MCM2 has the potential to serve as a novel cell proliferation biomarker in OSCC and VC when compared to cyclin D1.
