ABSTRACT
The cell cycle transcriptional program imposes order on events of the cell-cycle and is a target for signals that regulate cell-cycle progression, including checkpoints required to maintain genome integrity. Neither the mechanism nor functional significance of checkpoint regulation of the cell-cycle transcription program are established. We show that Nrm1, an MBF-specific transcriptional repressor acting at the transition from G(1) to S phase of the cell cycle, is at the nexus between the cell cycle transcriptional program and the DNA replication checkpoint in fission yeast. Phosphorylation of Nrm1 by the Cds1 (Chk2) checkpoint protein kinase, which is activated in response to DNA replication stress, promotes its dissociation from the MBF transcription factor. This leads to the expression of genes encoding components that function in DNA replication and repair pathways important for cell survival in response to arrested DNA replication.
Subject(s)
DNA Replication/physiology , DNA, Fungal/metabolism , G1 Phase/physiology , Repressor Proteins/metabolism , S Phase/physiology , Schizosaccharomyces pombe Proteins/metabolism , Schizosaccharomyces/metabolism , Checkpoint Kinase 2 , DNA Repair/physiology , DNA, Fungal/genetics , Genome, Fungal/physiology , Phosphorylation , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Repressor Proteins/genetics , Schizosaccharomyces/genetics , Schizosaccharomyces pombe Proteins/geneticsABSTRACT
This paper sums up the results of controlled field clinical trials of adult and childhood variants of Harvix, inactivated vaccine from hepatitis A. The vaccine is weakly reactogenic and safe. After a single injection of Harvix-1440 vaccine to adults the percentage of seroconversions in previously seronegative individuals was 80.6%, with the mean geometrical antibody titer 119 mIU/ml. In children aged 3-10 years the percentage of seroconversions after a single injection of Harvix-720 vaccine was 100%, the mean geometrical of antibody titer being 427 mIU/ml. Results of laboratory control and field clinical trials recommend both variants of Harvix vaccine for prevention of hepatitis A in the Russian Federation.
Subject(s)
Hepatitis A Vaccines/adverse effects , Hepatitis A Vaccines/immunology , Hepatitis A/prevention & control , Vaccines, Inactivated/adverse effects , Vaccines, Inactivated/immunology , Child , Child, Preschool , Controlled Clinical Trials as Topic , Hepatitis Antibodies/blood , Hepatovirus/immunology , Humans , PlacebosABSTRACT
The nucleotides sequence has been determined for the viral RNA and some of its cDNAs coding for the major antigenic protein of the epidemic stomatitis O1-194 and O1-1618 VP1 viruses. The expressed microheterogeneity has been registered for the population of the strain O1-194.
Subject(s)
Aphthovirus/genetics , Capsid/genetics , Genes, Viral , Amino Acid Sequence , Animals , Antigens, Viral/genetics , Aphthovirus/immunology , Base Sequence , Capsid/immunology , Capsid Proteins , Cloning, Molecular , DNA, Viral/genetics , DNA-Directed DNA Polymerase/genetics , Epitopes/genetics , Molecular Sequence Data , RNA, Viral/genetics , RabbitsABSTRACT
The nucleotide sequence of the cDNA for the viral RNA region coding for the main antigenic protein of the epidemic stomatitis virus of Asia 1 serotype has been identified. The amino acid sequences in the regions of VP1 protein antigenic determinants of the serotype Asia 1 virus and other serotypes viruses have been compared.