An investigation into the use of riverine mesocosms to analyse the effect of flow velocity and recipient textiles on forensic fibre persistence studies.

Textile fibre evidence can provide important activity level information in criminal cases. To date, very few studies have investigated fibre persistence on fabrics exposed to aquatic conditions, even though items of evidence and victim's bodies can regularly be found in aquatic environments. This lack of research on whether fibres (and other trace evidence) persist on evidence submerged in water, has shown to impact practice as it is reported that crime scene examiners do not attempt to recover this evidence, due to the belief that it would not be present. The dynamic nature of aquatic environments mean that the studies are difficult to conduct in situ and variables, such as water flow rate are not possible to control and thought to be difficult to monitor. To address these challenges, artificial streams (also known as mesocosms) were employed in this study to investigate the persistence rate of polyester fibres on different fabric types (Woollen/nylon mix carpet, 100% polyester fleece, and 95% polyester/5% elastane sports vest) for a four week exposure time (1, 8, 24, 48, 120, 168, 264, 336, 504 and 672 hrs). The effect of water flow rate on the persistence of fibres was investigated by conducting the experiment with two flow velocities; 'high' (∼2.75 L/s) or 'low' (∼0.7 L/s). Significant differences between textile type were seen at 504 hrs under low flow conditions and 8, 24, 168 and 264 hrs under high flow conditions. When comparing flow velocities, a significant difference was seen at 1 hr exposure for the fleece textile only, indicating that the two flow rates used in this study do not significantly affect fibre persistence. Initial loss rates were highest for the first hour of submergence for the carpet, fleece and sports vest. Fibre persistence rates were highest on the carpet, followed by fleece and then sports vest. Persistence rates remained mostly constant after 24 hrs for all textiles but with redistribution of fibres between textiles being seen after this exposure time. The use of artificial flumes in this study provided a balance between realistic experimentation and a controlled study; key experimental variables could be continously and safely monitored. This study provides the first fibre persistence data in river type environments and proposes a new method for testing persistence in aquatic environments. This approach is not limited to fibres evidence and could be employed for other evidence such as glass, pollen, fingerprints and DNA.

CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain.

Carbohydrate-active enzymes (CAZymes) are an important feature of bacteria in productive marine systems such as continental shelves, where phytoplankton and macroalgae produce diverse polysaccharides. We herein describe Maribacter dokdonensis 62-1, a novel strain of this flavobacterial species, isolated from alginate-supplemented seawater collected at the Patagonian continental shelf. M. dokdonensis 62-1 harbors a diverse array of CAZymes in multiple polysaccharide utilization loci (PUL). Two PUL encoding polysaccharide lyases from families 6, 7, 12, and 17 allow substantial growth with alginate as sole carbon source, with simultaneous utilization of mannuronate and guluronate as demonstrated by HPLC. Furthermore, strain 62-1 harbors a mixed-feature PUL encoding both ulvan- and fucoidan-targeting CAZymes. Core-genome phylogeny and pangenome analysis revealed variable occurrence of these PUL in related Maribacter and Zobellia strains, indicating specialization to certain "polysaccharide niches." Furthermore, lineage- and strain-specific genomic signatures for exopolysaccharide synthesis possibly mediate distinct strategies for surface attachment and host interaction. The wide detection of CAZyme homologs in algae-derived metagenomes suggests global occurrence in algal holobionts, supported by sharing multiple adaptive features with the hydrolytic model flavobacterium Zobellia galactanivorans. Comparison with Alteromonas sp. 76-1 isolated from the same seawater sample revealed that these co-occurring strains target similar polysaccharides but with different genomic repertoires, coincident with differing growth behavior on alginate that might mediate ecological specialization. Altogether, our study contributes to the perception of Maribacter as versatile flavobacterial polysaccharide degrader, with implications for biogeochemical cycles, niche specialization and bacteria-algae interactions in the oceans.