ABSTRACT
Mutagenic effect of asymmetric dimethylhydrazine (ADMH) on rats of different age groups upon acute and subacute treatment and protective effect of a Limonium gmelinii preparation. Genotoxic effect of ADMH depending on the dose and duration of treatment was established. The phytopreparation lacked mutagenicity and toxicity and had a protective effect in combination with the xenobiotic.
Subject(s)
1,2-Dimethylhydrazine/toxicity , Bone Marrow Cells/drug effects , Chromosome Aberrations , Environmental Pollutants/toxicity , Age Factors , Animals , Bone Marrow Cells/metabolism , Chromosome Aberrations/drug effects , Male , Mutagenicity Tests , Plant Preparations/pharmacology , Plant Preparations/toxicity , Plumbaginaceae , RatsABSTRACT
A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.
