Distribution of (3)H-dopamine and (3)H-DAGO binding sites in the central part of rat sinoatrial node.

Distribution of (3)H-dopamine and (3)H-DAGO binding sites was studied by autoradiography on semithin sections of total preparations of rat sinoatrial node. The relative density of (3)H-dopamine and (3)H-DAGO binding sites in the functional nucleus of the sinoatrial node was minimum and increased in the cranial and caudal directions. The level of (3)H-dopamine binding in the perinodal atrial myocardium was appreciably lower (22+/-6%), while binding of (3)H-DAGO was similar (76+/-16%) to that in the periarterial zone of the sinoatrial node.

Relative distribution densities of cholinergic and adrenoceptor structures in the central part of the sinoatrial node in rat heart.

Characteristics of distribution of cholinergic and adrenoceptor structures along the sinoatrial node artery in rat heart were evaluated by autoradiography on semithin sections by determining the density of (3)H-dihydroalprenolol and (3)H-quinuclidinyl benzilate binding sites. The relative density of binding sites for (3)H-dihydroalprenolol and (3)H-quinuclidinyl benzilate was minimum in the functional nucleus of the sinoatrial node and asymmetrically increased to maximum values to cranial (sharply) and caudal (smoothly) directions. The relative level of binding for (3)H-dihydroalprenolol in the perinodal atrial myocardium tissue was markedly lower than in the periarterial zone of the central part of the sinoatrial node and comparable to that for (3)H-quinuclidinyl benzilate.

Morphofunctional organization of sinoatrial node in rat heart.

The distribution of pacemaker cells along the sinus node artery was studied under conditions of short-term culturing using intracellular glass microelectrodes. The functional borders of the central and peripheral parts of the sinoatrial node were determined. The relationship between the position of the central part of the sinoatrial node and the patterns of the sinus node artery branching were analyzed.

Topography of 3H-DHA, 3H-QNB, 3H-dopamine, and 3H-DAGO binding sites distribution in the central part of the sinoatrial node in rat heart.

The topography of distribution of 3H-dihydroalprenolol, 3H-quinucledinyl benzilate, 3H-dopamine, and 3H-DAGO binding sites in the central part of the sinoatrial node in rat heart was studied by autoradiography after electrophysiological identification of the dominant pacemaker region location. Receptor asymmetry between the lateral and median regions of the central part of the sinoatrial node was shown. The dominant pacemaker region lay in the lateral area of the sinoatrial node; the number of binding sites for all four ligands was minimum in it. The number of binding sites gradually increased in the cranial and caudal directions from the dominant pacemaker region along the sinoatrial node artery (more smoothly in the caudal direction). The relative densities of bindings sites for 3H-dihydroalprenolol and 3H-dopamine were higher in the lateral region compared to the perinodal working myocardium, while the densities for 3H-quinucledinyl benzilate and 3H-DAGO were virtually the same. The distribution of binding sites along the artery in the median region of the sinoatrial node was even for 3H-quinucledinyl benzilate and 3H-DAGO. For 3H-DAGO these parameters were close to those in the perinodal atrial myocardium, for 3H-quinucledinyl benzilate somewhat lower. Curves presenting the distribution of binding site densities for 3H-dihydroalprenolol and 3H-dopamine in the median region of the sinoatrial node were similar, with a pronounced peak in the region contralateral to the dominant pacemaker region, and significantly higher binding parameters compared to those for the perinodal atrial myocardium. The difference consisted in higher density of 3H-dopamine binding sites in the median region of the sinoatrial node in comparison with the lateral region. Binding activity was maximum in the wall of the sinoatrial node artery. The distribution of binding sites for ligands to the main autonomic nervous system neurotransmitters in the rat heart sinoatrial node is heterogeneous.

[Treatment of bronchial obstruction in patients with pulmonary tuberculosis]. / Lechenie bronkhial'noi obstruktsii u bol'nykh tuberkulezom legkikh.

Whether the main points of treatment for bronchial obstructive syndrome (BOS) in chronic obstructive lung disease (COLD) can be adapted for patients with pulmonary tuberculosis (PT) was studied. For this purpose, 435 patients with PT with signs of BOS (forced expiratory volume at 1 second (FEV1) < 80% of the normal values) were examined. To establish differences in the efficiency of therapy, according to the activity of a process and to the magnitude of the impaired architectonics of the respiratory system, three main groups of observation were formed: 1) patients with infiltrative PT (IPT); 2) those with fibrocavernous PT (FCPT); and 3) those with posttuberculous pneumosclerosis (PS). According to the severity of BOS, the patients were divided into 3 subgroups: 1) 104 patients with FEV1 > 70% of the normal values; 2) 229 patients with FEV1 69-50%; 3) 102 patients with FEV1 < 50%. The patients with IPT and FCPT received the conventional antituberculous therapy under respective clinical, laboratory, and X-ray control. The patients with PS had no antituberculous therapy. All the patients underwent 3-month clinical and functional monitoring evaluating changes in life quality, by using the respiratory questionnaire of the Saint George hospital. The findings have led to the conclusion that the use of the proposed therapy for bronchial obstructive syndrome in patients with pulmonary tuberculosis was highly effective, promotes the amelioration of the degree of respiratory symptoms in patients with IPT by 2 to 8 times, in those with FCPT by more than 2-3 times, and in those with PS by 1.45-10 times. The differences in the efficiency of bronchodilator therapy depend on the baseline level of bronchial obstruction. In patients with pulmonary tuberculosis concurrent with BO, the use of current inhalation bronchodilator therapy results in a substantial increase in FEV1, which differentiates BOS in PT from COLD. The use of the proposed therapy in the multimodality treatment of patients with pulmonary tuberculosis showed no statistically significant differences in the changes in the degree of X-ray symptoms while this therapy permits acceleration of abacillation in patients with IPT by 16.8% and in those with FCPT by 14.8%. Effective bronchodilator therapy considerably enhances life quality in patients. Thus, early systematic and long-term performance of the bronchodilator therapy, based on the principles of bronchodilator therapy for COLD, in patients with PT concurrent with BOS may substantially enhance the efficiency of treatment in this category of patients.

In vitro effect of acetylcholine on function of sinoatrial node in rat heart.

Electrophysiological parameters of true pacemakers in the sinoatrial node of rat heart were recorded intracellularly using glass microelectrodes. In 11 of 13 experiments acetylcholine in increasing doses did not induce migration of the dominant pacemaker region, while in two cases its minor migration upstream the sinus node artery was observed.

Electronic patient record for N.N. Burdenko Neurosurgical Institute: on the verge of implementation.

This presentation is the first report about development of Electronic Patient Record System (EPRS) for N.N. Burdenko Neurosurgical Institute (NSI). This EPR system is the core of Integrated Automatic Information System intended to support all business processes running in the Institute. A new technology for developing information systems in poorly formalized subject domains, named IBS/Records, was was created.

[The characteristics of the histological structure of parasagittal meningiomas and their effect on postoperative recurrence]. / Osobennosti gistologicheskoi struktury parasagittal'nykh meningiom i ikh vliianie na posleoperatsionnoe retsidivirovanie.

Among 506 parasagittal meningiomas, 3 histological types: typical, atypical, and anaplastic ones were identified, which differ in malignancy, as evidenced by varying incidence rates of postoperative recurrences in these groups, and amounted to 11, 34.7, and 100%, respectively. The likelihood of postoperative recurrences of parasagittal meningiomas are substantially affected by the histological structural features, such as nuclear polymorphism, dense cell foci, papillary structures, mitoses, lymphoid infiltrates, and necrotic foci. The presence of meningiomas of perivascular lymphoid infiltrates in the structure may be regarded as an indicator of the benign biological behavior of a tumor while that of lymphoid infiltration in the meningiomal stroma considerably enhances the likelihood of tumor recurrence and is a poor predictor.