ABSTRACT
Morphological and genetic data have been obtained for five new East Asian species of the Psilostomatidae. The life cycle of Psilotrema limosum n. sp. was carried out using Parafossarulus manchouricus as the first intermediate host. Compared with the East Asian species of the genus, these worms differ in morphometric characters in both the cercarial and adult stages. Its validity was also confirmed by the 28S rRNA gene data. Data on the life cycle and morphology of developmental stages of Sphaeridiotrema ussuriensis n. sp. and Sphaeridiotrema aziaticus n. sp. were also obtained. Cercariae of these species are found in Parafossarulus and Boreoelona snails, respectively. Sphaeridiotrema ussuriensis n. sp., like Sphaeridiotrema monorchis in China, has one testis, while S. aziaticus n. sp. has two testes. In addition, S. monorchis from Vietnam and Sphaeridiotrema spinoacetabulum from the Russian southern Far East are justified as belonging to the new species named Sphaeridiotrema vietnamensis n. sp. and Sphaeridiotrema pyriforme n. sp., respectively. This proposition is based on the morphology of developmental stages, the list of the first intermediate hosts and the 28S rRNA gene data. Analysis of the phylogenetic relationships within Psilostomatidae revealed three clusters, including taxa with different life cycles strategies. Sphaeridiotrema was also divided into two groups, which combine species according to their intermediate hosts and geographical localisation.
