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2.
Biull Eksp Biol Med ; 83(5): 625-7, 1977 May.
Article in Russian | MEDLINE | ID: mdl-884278

ABSTRACT

Uridine-3H (2 muCi/g of body weight) was injected intraperitoneally to albino mice; this was followed (in one hour) by isopropylnoradrenaline (0.1 mg/g of body weight). The animals were sacrified in 10 minutes. Autoradiographic analysis and polarisation-microscopic study of the myocardial section showed that myokardiocytes with a lower level of the RNA synthesis were to the contracture lesions.


Subject(s)
Heart Diseases/pathology , Myocardium/pathology , RNA/biosynthesis , Animals , Heart Diseases/metabolism , Isoproterenol , Male , Mice , Mice, Inbred Strains , Myocardium/metabolism
3.
Tsitologiia ; 18(1): 102-5, 1976 Jan.
Article in Russian | MEDLINE | ID: mdl-941267

ABSTRACT

The possibility of employing a mixed suspension of isolated liver cells from several rats for estimation of some quantitative characters commonly used in cytological investigations was studied. It is demonstrated that the disaggregation rate of liver in different animals varies slightly, and the mean values of the parametres studied (the proportion of dividing and binucleated cell) obtained from individual animals coincides well with those of the mixed suspension.


Subject(s)
Liver/cytology , Animals , Cell Separation , Mitosis , Rats
4.
Ontogenez ; 7(3): 299-303, 1976.
Article in Russian | MEDLINE | ID: mdl-1026878

ABSTRACT

The proliferation of binucleated cells in the liver of young Wistar rats after partial (2/3) hepatectomy was studied by means of autoradiography and cytophotometry. The analysis of the kinetics of 3H-thymidine labelled cells has shown that both the bi- and mononucleated cells proceed through the mitotic cycle and enter mitosis simultaneously. The nuclei of 2nX2 cells enter prophase simultaneously but fuse during metaphase, so that the subsequent division results in the formation of mononucleated tetraploid cells.


Subject(s)
Cell Nucleus , Chromosomes , Liver Regeneration , Liver/cytology , Polyploidy , Animals , Autoradiography , Isotope Labeling , Male , Mitosis , Photometry/methods , Rats , Thymidine , Tritium
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