ABSTRACT
Several novel, differentially transcribed genes were identified in one centroblastic and one immunoblastic HIV-associated B-cell non-Hodgkin's lymphoma (B-NHL) by subtractive cloning. In both lymphomas, we detected an upregulated transcription of several mitochondrial genes. In the centroblastic B-NHL, we found a high level transcription of nuclear genes including the interferon-inducible gene (INF-ind), the immunoglobulin light chain gene (IgL), the set oncogene, and several unknown genes. The data obtained on upregulated expression of the genes in human B-NHL of HIV-infected patients considerably overlap with those obtained earlier for the B-NHL of simian immunodeficiency virus-infected monkeys. In the centroblastic lymphoma, one transcript revealed a fusion of the 3'-untranslated region of the set gene and the C-terminal region of the IgL gene. This chimeric sequence was confirmed by a site-directed polymerase chain reaction performed with total cDNA and genomic DNA. The expected amplification product was obtained in both cases pointing to a genomic rearrangement. The IgL-set fusion sequence was not found in cDNA preparations and genomic DNA of the immunoblastic HIV-associated B-NHL. Further studies are necessary to determine whether these genes contribute to lymphoma development or can be used as therapeutic targets.
Subject(s)
Lymphoma, AIDS-Related/metabolism , Lymphoma, Non-Hodgkin/virology , RNA, Messenger/metabolism , Transcription, Genetic , 3' Untranslated Regions , Base Sequence , Blotting, Northern , Blotting, Southern , Cloning, Molecular , DNA, Complementary/metabolism , Databases, Factual , Dose-Response Relationship, Drug , Humans , Immunoblotting , Immunoglobulins/metabolism , Lymphoma/metabolism , Molecular Sequence Data , Polymerase Chain Reaction , Sequence Homology, Nucleic Acid , Up-RegulationABSTRACT
Mitochondrial genes that are overexpressed in human and monkey B-cell non-Hodgkin lymphomas (B-NHLs) were sought via subtraction hybridization, cloning, and differential screening of the resulting cDNA libraries. The cDNAs of mitochondrial genes made an appreciable proportion of all lymphoma-specific cDNAs. Lymphomogenesis was associated with overexpression of a mitochondrial gene set which varied with lymphoma type and always included NADHIV. A possible association between overexpression of certain mitochondrial genes and cell malignant transformation is discussed.
Subject(s)
Lymphoma, B-Cell/genetics , Mitochondria/genetics , Transcription, Genetic , Animals , Cloning, Molecular , DNA, Complementary , Haplorhini , Humans , Nucleic Acid Hybridization , Subtraction TechniqueSubject(s)
Acquired Immunodeficiency Syndrome/complications , Lymphoma, AIDS-Related/genetics , RNA, Messenger/metabolism , Base Sequence , Cloning, Molecular , DNA, Complementary , HIV-1 , Humans , Lymphoma, AIDS-Related/complications , Molecular Sequence Data , Nucleic Acid Hybridization , RNA, Messenger/genetics , Sequence Homology, Nucleic Acid , Subtraction TechniqueABSTRACT
A 29.5 kda outer membrane protein (OmpB) of R. prowazekii virulent Breinl strain is known to differ from its counterpart in attenuated Madrid E strain, while OmpB of this latter one and of its virulent variant EVir coincide in mobility. The infectivity of these strains for macrophages was previously shown to be different as well, and to correlate with their virulence. Previously cloned R. prowazekii Breinl strain, 1.644-bp insert from lambda gtll recombinant expressing as OmpB in inducer-independent fashion was sequenced and used as a query to search for similarity in non-redundant GenBank/EMBL/DDBJ Data Base aided by BLASTX mail server. Extensive homology of inferred 282-aa sequence to peptidyl-propyl cis/trans isomerase C (PPIase C) of E. coli belonging to parvulin family of rotamases (foldases), and related proteins such as Campylobacter jejuni cell binding factor 2 (Cbf2), B. subtilis PrsA, and Lactococcus lactis PrtM has been revealed.