Glyphosate residues in soil can modify plant resistance to herbivores through changes in leaf quality.

Glyphosate is the most widely used non-selective herbicide in the world. Glyphosate residues in soil can affect plant quality by modifying plant physiology, hormonal pathways and traits, with potential consequences for plants' interactions with herbivores. We explored these indirect effects in the context of plant-herbivore interactions in a perennial, nitrogen-fixing herb. We quantified leaf herbivory for glyphosate-exposed and control plants grown in phosphorus-fertilized and non-fertilized soils, and assessed the impacts of glyphosate treatment on traits related to plant resistance against herbivores (leaf trichome density, leaf mass per area) and performance (aboveground biomass, root:shoot ratio, nodule number, nodule activity). Moreover, we conducted a laboratory feeding experiment to compare the palatability of leaves from glyphosate-exposed and control plants to a generalist mollusc herbivore. Herbivore damage and intensity in situ increased during the growing season regardless of glyphosate or phosphorus treatment. Glyphosate treatment reduced leaf trichome density but had no effect on the other plant traits considered. Herbivore damage was negatively associated with leaf trichome density. The feeding experiment revealed no difference in the feeding probability of mollusc herbivores between glyphosate-exposed and control plants. However, there was an interaction between glyphosate treatment and initial leaf area for leaf consumption by herbivores: leaf consumption increased with increasing leaf area in both groups, but at a lower rate for glyphosate-exposed plants than for control plants. Our results show that glyphosate residues in soil have the potential to indirectly affect aboveground herbivores through changes in leaf quality, which may have mixed consequences for folivore damage.

Population-wide shifts in herbivore resistance strategies over succession.

As a strategic cost-saving alternative to constitutive resistance, induction of resistance against herbivores in plants can be especially beneficial when enemies are scarce or variable in abundance. Although probably describing the two ends of a continuum, constitutive and induced resistance strategies have long been observed to trade off within species. Examining these traits among populations along a successional gradient can help explain how temporally variable environments can maintain genetic variation and how ecosystem processes are affected by shifting plant resistance trait expression over time. Here we leverage large experimental plots that represent a chronosequence of succession up to 15 yr in combination with common garden experiments to examine changes in the selective environment and genetic differences in tall goldenrod's (Solidago altissima) constitutive and induced resistance. We show that resistance against a specialist herbivore Trirhabda virgata was inducible in the plants originating from midsuccession, which coincides with the largest loads of herbivores. The flavonoid compound content of the leaves varied with successional stage of the population of origin, which is indicative of constitutive differences in secondary metabolite production. Finally, there was a clear trade-off between constitutive and induced resistance. Our study indicates that selection for resistance traits within a population can be highly variable over time and likely result in genetically determined shifts of resistance strategies over relatively short time periods via genotype sorting.