ABSTRACT
We studied the effect of intraperitoneal administration ACTH(4-7)-PGP in doses of 5, 50, 150, and 450 µg/kg to Wistar male rats 12-15 min before modeling restraint stress on the morphofunctional state of the colon. In rats exposed to restraint stress, signs of atrophy and inflammatory reaction in the colon wall, changes in functional activity and number of mast cells, and increased serum level of corticosterone were observed. Administration of the peptide led to a decrease in corticosterone concentration, alleviated stress-induced pathomorphological changes, and promoted adaptation of the intestinal wall to stress. The positive effects of ACTH(4-7)-PGP can be determined by multifunctional nature of the physiological and pharmacological effects of the neuropeptide.
Subject(s)
Adrenocorticotropic Hormone/analogs & derivatives , Peptide Fragments/pharmacology , Restraint, Physical/adverse effects , Adrenocorticotropic Hormone/blood , Adrenocorticotropic Hormone/pharmacology , Animals , Colon/drug effects , Colon/metabolism , Corticosterone/blood , Male , Rats , Rats, Wistar , Stress, Physiological/drug effectsABSTRACT
We studied the effect of Semax on the state of intestinal microbiota in rats subjected to restraint stress. Semax was injected to Wistar male rats intraperitoneally in doses of 5, 50, 150, 450 µg/kg 12-15 min before modelling chronic restraint stress. It was found that stress exposure reduced the number of obligate bacteria in the colon microbiota, but increased the content of opportunistic microorganisms. Semax in doses of 50 and 150 µg/kg prevented the stress-induced changes in the composition of colon microbiota. The observed effects of Semax might be mediated by the central neurotropic effects as well as by binding to peripheral melanocortin receptors of the intestine.
Subject(s)
Adrenocorticotropic Hormone/analogs & derivatives , Colon/drug effects , Colon/microbiology , Gastrointestinal Microbiome/drug effects , Peptide Fragments/therapeutic use , Adrenocorticotropic Hormone/therapeutic use , Animals , Disease Models, Animal , Male , Rats , Rats, Wistar , Restraint, Physical , Stress, Physiological/drug effectsABSTRACT
We studied the effects of Selank on the condition of the colon wall in Wistar male rats subjected to restraint stress. Selank was injected intraperitoneally in doses of 80, 250, and 750 µg/kg 15 min before stress exposure. In rats subjected to stress, signs of atrophy, inflammatory reaction, and changes in the number and functional activity of mast cells were observed against the background of increased corticosterone level. Selank administration led to a decrease in corticosterone levels, reduced pathomorphological manifestations of stress exposure, and accelerated adaptation. These effects were presumably realized due to multifunctional biological effects of Selank.
Subject(s)
Oligopeptides/therapeutic use , Animals , Colon/drug effects , Colon/metabolism , Corticosterone/metabolism , Disease Models, Animal , Male , Mast Cells/drug effects , Mast Cells/metabolism , Rats , Rats, Wistar , Restraint, Physical , Stress, Physiological/drug effectsABSTRACT
We studied the effects of Selank on intestinal microbiota in Wistar male rats subjected to chronic restraint stress. Selank was injected intraperitoneally in doses of 80, 250 and 750 µg/kg 15 min before stress exposure. Chronic restraint stress led to a decrease in the content of obligate microflora, while the content of opportunistic microorganisms increased. Selank restored intestinal microbiota presumably via central (neurotropic) and peripheral (immunotropic) mechanisms.
Subject(s)
Colon/microbiology , Gastrointestinal Microbiome/drug effects , Oligopeptides/therapeutic use , Restraint, Physical/physiology , Animals , Disease Models, Animal , Male , Rats , Rats, WistarABSTRACT
AIM: Study the possible qualitative and quantitative changes of microbial community of the parietal mucin of the large intestine and the state of the wall of the large intestine in experimental animals underbackground and anomalous influence of geomagnetic field. MATERIALS AND METHODS: CBA mice were put under the influence of anomalous magnetic field comparable to its intensity in Zheleznogorsk (3 Oe) for 1 and 2 weeks. Quantitative and qualitative study of mucous microflora of the large intestine of the mice was performed by bacteriological method. Identification of the microorganisms was performed by microbiological analyzer "Multiskan-Ascent" and commercial test-systems "Lachema-Czech Republic": ENTHEROtest-16, STAPHYtest-16, Streptotest-16, En-COCCUStest-16; for lactobacilli and bifidobacteria identification - API 50 CHL (bioMerieux). Bacteria content in 1 g of material was calculated by the number of microorganism colonies grown. RESULTS: A pattern of changes of mucous microflora of the intestine and the state of the wall of the large intestine of the experimental animals that had been put under the influence of anomalous magnetic field is shown. During evaluation of qualitative and quantitative diversity of microbial community of parietal mucin of the large intestine of the mice under the influence of magnetic field on the background and anomalous levels changes not only in quantity and frequency of detection of obligate, transitory flora but also cell elements of mucous membrane of the wall of the large intestine were established. CONCLUSION: The results of the study allow to make a conclusion about the presence of reactivity of the parietal microflora of the intestine of the mice to the influence of the anomalous magnetic field. This leads to changes in cell elements in the mucous membrane of the wall that manifest by infiltration of the connective tissue stroma by leucocytes and reconstruction of epithelium, that are features of dysbiosis.
Subject(s)
Gram-Negative Bacteria/radiation effects , Gram-Positive Bacteria/radiation effects , Intestinal Mucosa/microbiology , Intestine, Large/microbiology , Magnetic Fields/adverse effects , Metagenome/physiology , Animals , Apoptosis/radiation effects , Bacterial Typing Techniques , Colony Count, Microbial , Electromagnetic Radiation , Gram-Negative Bacteria/growth & development , Gram-Positive Bacteria/growth & development , Granulocytes/radiation effects , Histocytochemistry , Intestinal Mucosa/metabolism , Intestinal Mucosa/pathology , Intestinal Mucosa/radiation effects , Intestine, Large/metabolism , Intestine, Large/pathology , Intestine, Large/radiation effects , Mice , Mice, Inbred CBA , Mucins/chemistry , Mucins/metabolism , Mucus/chemistry , Mucus/metabolism , Neutrophil Infiltration/radiation effectsABSTRACT
Increase in incidence of candidal colpitis has been observed during use of contraceptive drugs. Elimination of staphylococci from genital tract after use of contraceptives was detected in 34,7% of patients. Composition of other aerobic microflora did not change. Nonspecific immune reaction was characterized by intensified phagocytosis, increase of results of NBT reduction test and level of proinflammatory cytokines. Conclusion about inappropriateness of using low-dose oral contraceptives in patients with recurrent vulvovaginal candidosis was made. Such contraceptives can be recommended to women with prolonged inflammatory diseases of reproductive system.
Subject(s)
Contraceptives, Oral, Hormonal/adverse effects , Vaginitis/immunology , Vaginitis/microbiology , Candida/isolation & purification , Candidiasis/chemically induced , Candidiasis/immunology , Candidiasis/microbiology , Candidiasis/prevention & control , Contraceptives, Oral, Hormonal/administration & dosage , Cytokines/biosynthesis , Female , Humans , Immunoglobulin A/isolation & purification , Immunoglobulin A, Secretory/isolation & purification , Immunoglobulin G/isolation & purification , Peroxidase/metabolism , Phagocytosis , Staphylococcal Infections/drug therapy , Staphylococcus/isolation & purification , Vagina/immunology , Vagina/microbiology , Vaginitis/chemically induced , Vaginitis/prevention & controlABSTRACT
Experimental Salmonella infection in mice, developing simultaneously with the prolonged action of an artificial constant magnetic field with induction equal to 3 x 10(-4) T, was found to induce a pronounced decrease in nonspecific resistance in the animals. The study of Salmonella population structure revealed that the cells selected the animals subjected to the action of the artificial magnetic field had mostly a lesser number of signs of antibiotic resistance. By the end of the experiment Salmonella cultures isolated from the mice subjected to the action of the artificial magnetic field were characterized by greater virulence and resistance to the bactericidal action of blood serum. The use of sodium nucleinate under the conditions of the action of the artificial magnetic field enhanced the level of anti-infectious protection in the animals, which changed the direction of cell selection in Salmonella population towards cells with a greater number of markers of antibiotic resistance.
Subject(s)
Magnetics/therapeutic use , Salmonella Infections, Animal/therapy , Salmonella typhimurium , Adjuvants, Immunologic/therapeutic use , Animals , Drug Resistance, Microbial , Drug Resistance, Multiple , Immunity, Innate , Mice , Mice, Inbred C57BL , Nucleic Acids/therapeutic use , Salmonella Infections, Animal/immunology , Salmonella Infections, Animal/microbiology , Salmonella typhimurium/isolation & purification , Salmonella typhimurium/pathogenicity , Time Factors , VirulenceABSTRACT
The passage of salmonellae through mice with different immune status (intact, immunosuppressed and immunized) has been found to lead to the multidirectional selection of the animals. Passages through intact and immunosuppressed mice lead mainly to the selection of the animals with the minimal number of resistance markers, while passages through immunized mice result in the selection of the animals with the maximal number of the markers of resistance to antibiotics. The ratio of these kinds of cells coincides with the degree of the virulence of the population: salmonellae isolated from immunosuppressed mice have the highest virulence and those isolated from immunized mice have the lowest virulence.
