ABSTRACT
In the present article, a novel and effective ZnO quantum dots-based fluorescent probe has been developed for the detection of cysteine in different solutions. Firstly, melamine-based fluorescent pre-probe was successfully synthesized via condensation reaction and, then ZnO quantum dots (QDs) were homogenously dispersed into this solution. This fluorescent probe was used for the detection of cysteine in different solutions such as bovine serum albumin and tap water. ZnO QDs were characterized using XRD, nano-particle size analyzer, and FE-SEM techniques. The size of the ZnO QDs was calculated as 28.03±9.86 nm, and 31.95±10.02 nm from Scherrer's equation and nano-particle size analyzer, respectively. The developed fluorescent probe was exhibited a highly selective and sensitive response to the detection of cysteine. Also, the proposed fluorescent probe has a larger Stokes shift value (236 nm). The limit of detection and linear range of ZnO QDs-based fluorescent biosensor were found as 0.642 µM and 0.1-600 µM, respectively. ZnO quantum dot-based fluorescent sensor for L-cysteine.
Subject(s)
Biosensing Techniques , Cysteine/analysis , Fluorescent Dyes/chemistry , Quantum Dots/chemistry , Zinc Oxide/chemistry , Fluorescent Dyes/chemical synthesis , Hydrogen-Ion Concentration , Molecular Structure , Particle Size , Spectrometry, FluorescenceABSTRACT
In the present paper, a highly selective and sensitive fluorescent biosensor based on poly(azomethine-urethane) and zeolite for the determination of DNA molecules was developed. Zeolite was chosen to enhance with anionic or cationic functional groups in polymer matrix and interaction between polymer and DNA. Several parameters such as polymer concentration, pH and incubation time effect on the sensitivity of the fluorescent biosensor were optimized. Linear range was determined between 2.50 and 25.00â¯nmol/L DNA concentration and limit of detection (LOD) of the biosensor was calculated as 0.095â¯nmol/L under the optimal conditions. Interference study was also performed in the presence of different amino acids, cations and organic compounds. The results clearly indicated that the tested cations and compounds were not induced a significant fluorescence change and the proposed zeolite-based biosensor was shown a good selectivity for DNA.
