ABSTRACT
Poly (lactidecoglycolide) (PLGA) nanoparticles (NPs) are biodegradable carriers that participate in the transport of neuroprotective drugs across the blood brain barrier (BBB). Targeted brainderived neurotrophic factor (BDNF) delivery across the BBB could provide neuroprotection in brain injury. We tested the neuroprotective effect of PLGA nanoparticlebound BDNF in a permanent middle cerebral artery occlusion (pMCAO) model of ischemia in rats. SpragueDawley rats were subjected to pMCAO. Four hours after pMCAO, two groups were intravenously treated with BDNF and NPBDNF, respectively. Functional outcome was assessed at 2 and 24 h after pMCAO, using the modified neurologic severity score (mNSS) and rotarod performance tests. Following functional assessments, rats were euthanized blood was taken to assess levels of the neurobiomarkers neuronspecific enolase and S100 calciumbinding protein ß (S100ß), and the brain was evaluated to measure the infarct volume. The NPBDNFtreated group showed significant improvement in mNSS compared with pMCAO and BDNFtreated groups and showed improved rotarod performance. The infarct volume in rats treated with NPBDNFs was also significantly smaller. These results were further corroborated by correlating differences in estimated NSE and S100ß. NPBDNFs exhibit a significant neuroprotective effect in the pMCAO model of ischemia in rats.