[Nutriome as the direction of the "main blow": determination of physiological needs in macro- and micronutrients, minor biologically active substances].

One of the essential parts of fundamental research in Nutrition Science is the determination of the physiological requirements of humans for energy and food substances. Research that has been carried out in this area over the past 90 years, consistently develops and improves the norms of physiological requirements for energy and nutrients for various groups of the population of the Russian Federation. In the 50 years of the last century in this research field, determining the values of daily intake for macronutrients (proteins, lipids and carbohydrates), was in the first place. Then the Era of micronutrients (vitamins, minerals, trace elements) was started, and, finally, now there is the Era of minor food biologically active substances. More and more facts are accumulating about their leading role in regulating metabolism. They can be recognized as endogenous regulators, the primary vital components involved in the formation of human health. In recent years, the new definition of Nutriome is introduced into Nutrition Science. It is considered as a set of essential nutritional factors to maintain a dynamic equilibrium between human being and the environment, aimed to ensure viability, the preservation and reproduction of the species, keeping the adaptive capacity, the system of antioxidant defence, apoptosis, metabolism, and immune system function. The Nutriome is a formula for optimal nutrition, which is continually being improved and supplemented. Knowledge of this formula is the key to forming an optimal diet for a person, and, therefore, to save their health. It is evident that at the population level, the Nutriome has its characteristics, its structure for each age period of human life. The need to develop a formula for optimal nutrition and, consequently, updating nutrient-based dietary guidelines is induced by socio-economic and demographic changes in population, changes in anthropometric characteristics of children and adults, increasing prevalence of socially significant non-communicable diseases, developing studies of the significance of particular food substances and establishing the relationship between nutrition and health.

[The transit of Russian nation nutrition at the turn of the 20th and 21st centuries].

The assessment of the individual dietary intake of the population acquired critical importance in the Russian Federation when implementing socio-economic reforms in the 1990s. This was due to the difficulties of the transition period from a planned economy to market relations, which was reflected in a decrease in the level of the socio-economic condition of the population. The purpose of this study was to analyze objective and reliable information about the nature of consumption of nutrients and energy, as well as food products by the adult population of Russia in the period 1994-2018. Material and methods. The paper analyzes and summarizes the results of sample surveys of household budgets conducted by the Federal State Statistics Service (Rosstat) in 1991-2018 and of large-scale epidemiological studies of the actual dietary intake of the population of the Russian Federation, which are presented by the data of the "Russian Longitudnal Monitoring Surveys" (RLMS) in 1994-2012 and large-scale nutrition surveys conducted by Rosstat in all constituent entities of the Russian Federation in 2013 and 2018. All nutrition surveys were carried out according to a unified methodology for collecting, processing and reporting actual data developed at the Federal Research Center of Nutrition, Biotechnology and Food Safety. Using a unified methodological basis allows us to build an analysis of the dietary intake of the population for the period from 1994 to 2018. Results. The objective data on the consumption of energy, nutrients, individual and aggregated groups of food products were analyzed depending on a number of socio-demographic and socio-economic variables that determine the nutrition pattern of the population of the Russian Federation during the period of economic reforms in the 1990s and 2000s. According to budget surveys, over the past years, the structure of the diet in households has changed significantly: the consumption of potatoes decreased by 45% and bakery products by 6%, the consumption of fish and fruits almost doubled, the consumption of meat products increased by 60%, of vegetables by 20%. According to epidemiological studies, the fat content increased from 31 to 36.8% of calorie content, the protein content didn't changed significantly, although in recent years there has been a tendency to increase to 12.1% of calorie content. The average per capita consumption of protein and fat by adults, including saturated fat acids were significantly higher in 2005-2018, compared with the 1990s, both in absolute quantities and in per cents of the caloric intake. At the same time, the consumption of total carbohydrates in per cents of the calorie intake and the amount of dietary fiber in the diet decreased. Parallel to fat intake changes, an increase in the prevalence of overweight and obesity in the adult population has been shown. A consistent decrease in adult consumption of added sugar in per cents of the total calorie intake was revealed from 14.0% in 1994 to 12.3-12.8% in 2012-2018. Changes in the structure of energy consumption and macronutrients are due to changes in the structure of the food ration set that have occurred over the years of observation. The total consumption of dairy products in terms of milk increased: the consumption of the amount of dairy products (drinks and "spoons"), curd products and cheeses increased, only the consumption of drinking milk did not change. In 2012 and 2013 consumption of meat products and sausages was significantly higher than in previous years. There was an increase in the consumption of fish, vegetables, fruits, fats and oils, juices in the 2000s compared with the 1990s. On the contrary, over the years of observation, a consistent decrease in the consumption of the total grain products was observed. These changes in the consumption of macronutrients and foods are a characteristic feature of the transition period from "poor" to "rich" dietary intake and the increase in 2000s in the diet energy density due to an increase in the proportion of fat and a decrease in the proportion of carbohydrates, which contribute to weight gain and an increase in the prevalence of overweight and obesity. The dependence of the structure of the diet on the economic situation of the family has been established, which implies the problem of malnutrition, both general malnutrition and insufficient intake of micronutrients (vitamins and minerals), in poor families. Conclusion. An assessment of the current nutritional status of the Russian population implies a double burden of disruption, determined both by the simultaneous presence of malnutrition problems and the increase in the prevalence of overweight and obesity.

[COVID-19: new challenges for medical science and practical health].

Currently, due to the wide spread of the new coronavirus infection COVID-19 and the need for anti-epidemic measures, medical science should integrate all efforts to ensure, on the one hand, at the global level - the fight against the spread of infection, on the other hand, at the individual level - increasing the adaptive capacity and immune response of the organism to protect against COVID-19. Nutrition is the most important factor determining human health and the functioning of all mechanisms to protect a person from negative environmental factors. For the prevention and treatment of new coronavirus infection COVID-19, a significant role is played by the correction of eating disorders, including vitamin and micronutrient deficiency. Federal Research Centre of Nutrition and Biotechnology, together with Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor), prepared Recommendations on nutrition for children and adults, requiring a self-isolation regime or quarantine at home in connection with COVID-19. In order to promptly inform and advise the population on nutrition optimization in the early days of anti-epidemic measures, an information reference Contact Center was created and is continuously functioning on the basis of the Federal Research Centre of Nutrition and Biotechnology. Equally important is the implementation of measures aimed at the prevention of food contamination with COVID-19 agent. In this regard, the Federal Research Centre of Nutrition and Biotechnology, together with Rospotrebnadzor prepared guidelines on measures to prevent the transmission of a new coronavirus infection through foods. Another aspect that the medical community has faced in connection with the pandemic is the need to reorient specialized medical organizations that have not previously worked with patients with infectious diseases in order to create Clinical centers for the treatment of the new coronavirus infection COVID-19. Thus, in the context of the spread of the new coronavirus infection COVID-19, many areas of medicine are involved in the process of providing anti-epidemic measures. Successful completion of the tasks will significantly reduce the negative consequences of the COVID-19 pandemic for the state and citizens.

[Analysis of the association of diet energy from macronutrients and prevalence of overweight and obesity among the Russian population].

The fundamental cause for the development of overweight and obesity in adults is a positive balance of energy. However, many environmental factors can contribute to or restrain the development of obesity. These, of course, include the composition of the diet and the structure of its energy value. The aim of the study was to explore the relationship between the prevalence of overweight and obesity with the macronutrients' energy intake of Russian adult population. Material and methods. Assessment of dietary intake and anthropometric parameters (height, weight) of the adult population were obtained in a cross-sectional survey of households in Russia (Russian longitudinal monitoring surveys, RLMS project). For analysis, we selected data from a survey of adults of both sexes (24 767 men and 30 040 women) aged 19-60 years, obtained in 7 rounds of the project during the period 2000-2012. Association of dependent variables of macronutrients' energy as a percentage (%E) (fat, protein, total carbohydrates, natural mono- and disaccharides, added sugar), and independent (factor) variables namely gender and 4 categories of body mass index (BMI), characterizing nutritional status, was investigated using the ANOVA. The total daily energy intake and age were used as covariates in the model. Results and discussion. The intake of total daily energy, the absolute values of macronutrients' intake and %E of macronutrients are significantly higher in obesity (with the exception of the consumption of various fractions of carbohydrates) compared to normal BMI values. A direct association of the %E protein and the BMI category has been established. This relationship is more pronounced in men, but statistically significant in women as well (p<0.001 when comparing all BMI groups). A linear increase in the proportion of %E of fat with an increase in the BMI category in the direction from malnutrition to obesity was found in men (p<0.001 when comparing all BMI groups). In women, statistically significant differences in %E of fat intake in BMI groups were observed only between obesity and normal. In men, the consumption of %E due to total carbohydrates is lower in groups with overweight and obesity compared with normal and malnutrition. The exception is only for %E of natural mono- and disaccharides, the consumption of which in men increases in the range from malnutrition to obesity. In women with overweight and obesity, consumption of %E due to polysaccharides and natural sugar doesn't differ from consumption in persons with normal BMI. Consumption of %E due to added sugar and the frequency of consumption of more than 10%E due to sugar are reduced in people of both sexes in the direction normal, overweight, and obesity. The opposite effect of BMI on the consumption of %E of fat and carbohydrates is clearly manifested in an increase in the ratio of %E fat/%E carbohydrates in people with overweight and obesity. Analysis of variance was confirmed when assessing the frequency of distribution of respondents with overweight and obesity in quartiles of macronutrients' %E differentiated by gender. The frequency of overweight and obesity is significantly higher in the groups of highest quartiles of %E of protein, total fat, saturated fatty acids and lowest quartiles of %E of total carbohydrates and their individual fractions, including added sugar. Conclusion. The data obtained indicate a more significant role of the energy of the diet fat component in the prevalence of overweight and obesity, while the energy of carbohydrates, including added sugar, has no direct association with the prevalence of these conditions. The results obtained should be taken into account when developing measures to prevent and reduce the prevalence of obesity, both at the population and individual levels.

[Development and assessment of the reliability of the basic healthy eating index for the Russian population].

The conventional presentation of data on the actual average daily consumption of energy, nutrients, food products, as well as critically important risk factors complicates a holistic dietary assessment, consisting of at least a dozen indicators only for nutrients. The aim of the study was to develop a healthy eating index (HEI), based on epidemiological data on the actual nutrition of the Russian population and the criteria and principles of healthy nutrition adopted in the country. Material and methods. We used the data on the dietary intake of nutrients, energy and the main food groups obtained from the cross-sectional nutritional survey of members of 45 000 households over the age of 3 years conducted by Federal Statistics Service (Rosstat) in all regions of the Russian Federation in 2013. The criteria for scoring the indicator-components of the HEI were developed taking into account modern recommendations about the magnitudes of consumption of the most important food groups, as well as the levels of consumption of critical risk factors for chronic non-communicable diseases. Results and discussion. The HEI design included 5 indicators characterizing the adequacy of consumption of the main food groups, as well as 5 indicators of nutritional risk factors for chronic non-communicable diseases. Consumption of food groups (cereals, dairy, meat products, excluding sausages, vegetables, fruits) and added salt was calculated in g per 1000 kcal, and critical risk factors (total fat, saturated fat, added sugars) were estimated as a percentage of total energy. As a result of transformations of quantitative variables of consumption of macronutrients (risk factors) and food products, quantitative scores were obtained (0-10 points) for all 10 indicator-components of the HEI. The assessment of the reliability of the HEI (validation) showed that a statistically significant positive correlation can be traced between the integral HEI and the indicators. This is a sign of reliability and objectivity of the HEI design. At the same time, a negative correlation was found between the value of the HEI and the scores of meat products, including sausages, that was the basis for excluding sausages from calculating the amount of meat products. It has been shown that HEI is significantly higher in females in age groups older than 11 years. A pronounced dependence of HEI on the age of people of both sexes has been established, that may be an indicator of the high sensitivity of HEI to changes in dietary patterns. The maximum HEI values were noted in children, the minimum - in adults 19-60 years, with a subsequent increase in the age group over 60 years. There were no changes in HEI in obese men, but HEI in women with body mass index (BMI) above 30.0 was significantly higher than in those with normal body weight (BMI 18.5-24.9). Conclusion. Further research is needed on the sensitivity of the HEI in terms of assessing the dietary patterns and dependence on socio-economic and other variables, including a wide range of dietary modifications.

[Dietary intake of calcium in pre-school and school children in Russia: main food sources and eating occasions].

It is known that adequate calcium intake determines formation of skeleton and genetic predisposition of its weight and density peak. Optimal calcium intake in the childhood determines risk reduction of density loss and osteoporosis in the adulthood. This research explored contribution of different food sources, as well as eating occasions on calcium dietary intake among preschool and school children (4-13 years old). Mean daily Calcium intake and dairy products consumption depending on family income have been analyzed. Dietary intake was evaluated by 24-hour recall method. National food composition data tables have been used to estimate mean nutrient intake. Dairy products were classified in accordance with local food grouping system. Percent of children consuming products - sources of calcium, amount per consumer, contribution of eating occasions to Calcium intake, foods consumed at breakfast as well as Calcium intake and dairy products consumption depending on household income has been analysed. Nationally representative sample of preschool and school age children, Russia National Nutritional Survey, was conducted in all regions of Russia in 2013 by state Federal Statistics Service - Rosstat. Sample size was 22 771 children split in four age groups: 4-6 y.o. (n=7303), 7-8 y.o. (n=4391), 9-10 y.o. (n=4691) and 11-13 y.o. (n=6386). The study confirmed the indications in the domestic and foreign literature that calcium intake was insufficient in children aged 4-13 years. Data showed age related particularities in consumption of products-sources of Calcium: percent of children consuming milk and milk-based beverages, milk porridges, cottage cheese declined rapidly in older age groups in comparison to preschoolers. A slight increase of cheese consumption was shown in older age groups. Breakfast is an important eating occasion that provides a significant proportion (23-28%) into calcium daily intake in children. Habit of skipping breakfast puts calcium daily intake at risk as it is not compensated throughout the day at other eating occasions. Our results indicate lower daily Calcium intake in breakfast skippers (11-14% of children) compared with kids of the same age who had breakfast at the day of survey. Sweetened tea and coffee consumption increased in older age groups and contrary, a milk and milk based beverages consumption droped in school age. We found lower Calcium intake and lower accessibility to variety of Calcium food sources in low socio-economic class of the children population, where milk seems to be the main product from milk and dairy food group. The findings can assist in developing food-based recommendations for optimal intake of calcium in children's daily diet. Consumption of dairy products is required to meet adequate intake and to low down prevalence of inadequate intake. There is room for improvement of dairy product consumption in terms of variety and number of serving a day especially, for children in older age groups. More attention to be dedicated to breakfast as it is an important eating occasion that contributes significantly to calcium daily intake. Study findings call for intervention programs aiming to correct the inadequacies of calcium intake in schoolchildren's diets, including nutrition education for families and support for accessibility of the products rich in calcium by low socio-economic population groups; for example, by inclusion of milk and dairy products into the school feeding programs.

[Actual liquid consumption by highly qualified athletes in the mode of the training process].

The purpose of the study was to evaluate the actual intake of fluids by athletes of various sports during the day with one and two training sessions before, during and after workout. The dietary intake, including consumption of various types of liquid foods and beverages, was evaluated by the method of 24-hour recall in 280 athletes of high qualification (candidates for masters and masters of sports) of both gender of various sports during the training period. It has been established that the main drink of rehydration was drinking bottled water. Bottled water was consumed on average by 86% of athletes. It was consumed by 95-96% of sportsmen from the group of single combats and power kinds, whereas in other groups the share of water consumers was less - 67-79%. In second place in terms of percentage of consuming was tea. Consumption of sports drinks was observed only during training by athletes from the group of cyclic sports (31%) and single combat (11%). Calculating the per capita fluid intake of athletes who had 2 workouts a day showed that athletes from the martial arts group consumed the largest volumes of fluid in the mode of both training sessions as compared to representatives of other sports. Athletes of other sports consumed on average less liquid in the 2nd training mode compared to the 1st one. The total fluid intake during two training sessions was maximum in the group of martial arts and was minimum in the group of complex coordination sports. It should be specially noted a small proportion of athletes who consumed specialized sports drinks - only 17% of athletes and more than half of them - cyclical sportsmen. Consumption of liquid food outside training has been observed in 76% of athletes. The mean volume of consumed liquid products varied by the user from 382 and 437 ml in complex coordination and game sports up to 504-553 ml in other sports. The daily fluid intake was maximum (2326 ml) in athletes engaged in martial arts, minimum (1009 ml) - in athletes of complex coordination sports.

[The study of nutrition, anthropometric tеstes and body composition among native and alien population of Russian Arctic].

Arctic zone refers to the territories that are uncomfortable for living and working of people. Ecological features of the Far North have contributed to the adaptation of the indigenous population to the conditions of the external environment, which manifested in the peculiarities of nutrition and nutritional status, which is also related to the presence of genetic polymorphisms in the population of the Arctic zone. The study of nutrition and the nutritional status of 180 people (78.9% woman и 21.1% man) living in the Far North was conducted. The data obtained indicate that the prevalence of overweight and obesity (62.7%) among the surveyed people living in the Arctic zone, was higher than among Russian people (average 57.3%). Indigenous people (mean age - 45.6±1.3 years) compared with alien population (45.1±2.6 years old) had lower BMI (27.2±0.5 vs 28.8±1.3 kg/m2) and a smaller hips along with significantly (p<0.05) lower relative fat body mass (31.9±0.9 vs 35.4±1.4 kg), lean (67.1±0.8 vs 64.6±0.9 kg) and bone body mass, as well as exchange at rest (1329.9±16.0 vs 1455.4±44.0 kcal). Indigenous population consumed significantly more bakery products and fish (1.5-2.2 fold), and less - dairy products and vegetables (1.7-2.0 fold). Thus, the peculiarities of the nutritional status of the indigenous and alien populations of the Arctic seem to be associated with the traditions of nutrition and lifestyle.

[The association of rs993609 polymorphisms of gene FTO and rs659366 polymorphisms of gene UCP2 with obesity among Arctic Russian population].

It is known that particular qualities of the prevalence of obesity, characteristic for the population of the Far North, are connected with the presence of genetic polymorphisms. The association of two polymorphisms (rs993609 of FTO gene and rs659366 of UCP2 gene) with obesity was studied in 175 people living on the territory of the Russian Arctic (Yamalo-Nenets Autonomous District). The incidence of obesity risk allele (A) of FTO gene rs9939609 polymorphism in the surveyed population of the Arctic (30.8%) was lower by 15% than that of the population of the central regions of Russia, Caucasian Americans and Europeans, but was higher by 18-20% than in Alaska inhabitants. The frequency of the A allele of the FTO gene was significantly 1.4-fold lower in indigenous Arctic people than in the alien population (p<0.05), that had significantly higher content of body fat (by 12%) than the indigenous population, including the Nenets (p<0.05). Рositive association had been identified between T allele rs659366 polymorphism of UCP2 gene and obesity risk in the surveyed who lived in the Arctic zone. Thus, the inhabitants of the Far North revealed the presence of genetic variants that contribute to heat production.

[Non-alcoholic beverages: russian priorities].

The article presents data of a survey of 11 850 persons of both sexes and various age groups (from 12 to 60 years) conducted in all Federal Districts of Russia, from 2013 to 2016. The analysis of consumption by the population of various widely consumed beverages, including different types of tea, low-calorie and dietary carbonated soft drinks and waters from various sources, was conducted and an analysis of the possible correlation between their frequency of consumption and body mass index (BMI) was carried out. It has been established that practically in all groups questioned about 10-15% of persons of various Federal districts and ages consume tap water. At the same time, the number of people consuming filtered, bottled water has significantly increased. In all age groups, except for a group of people aged 12-17 years, the consumption of tea by female obese women was 10-13% less than in the male group, and almost all groups with normal BMI consumed tea more often, than men. At the same time, there were no gender differences in the consumption of tea by individuals with a BMI of 25-30. There was no correlation between consumption of low-calorie, dietary carbonated soft drinks and BMI. Only in the group of people aged 31-45 years, gender differences were found, while in this group of people it was shown that obese men were about 3 times more likely than women to use these drinks (67.7 vs 25.0%).

[Vitamin status of rural residents, living in Russian Arctic].

The aim of this cross-sectional observational study was to estimate vitamin D, B2, A, E, ß-carotene (BC) status in rural residents, living in Russian Arctic (Yamal-Nenets Autonomous Area). 216 healthy adults (166 women, 50 men, 45.4±0.8 years old; BMI 27.5±0.5 kg/m2), 76% aboriginal ethnicity and 24% non-aboriginals subjects have been surveyed in spring 2016. Nenets were 72% among the surveyed, Russians - 14%, other ethnic groups - 14%. A total of 69 and 78% of subjects had 25(OH)D and BC blood serum concentrations less than the recommended target threshold of 30 ng/ml and 20 µg/dL respectively. Deficiency of vitamins B2, A and E, when blood serum concentrations of riboflavin <5 ng/ml, retinol <30 µg/dL and tocopherols <0.8 mg/dL, were found in 34, 15 and 13% of participants, respectively. The indigenous population was better supplied with vitamins D and B2. The lack of these vitamins was detected 1.3 and 1.8 fold less often (p<0.05) than in the non-aboriginals; whereas non-aboriginals were better provided with vitamin A and BC: the deficiency of these micronutrients was detected 3.1 and 1.8 fold less often (p<0.05) than in the inhabitants. In general, only 2.4% of those surveyed were sufficiently supplied with all 4 studied vitamins and BC. The frequency of deficit of one or two vitamins was revealed in 62.2%, combined deficiency of 3 or more vitamins was detected in 35.4%. There were no statistically significant differences in the incidence of combined multivitamin deficiencies among the indigenous and non-aboriginals. High incidence of vitamin A deficiency in the indigenous female population (21%) along with insufficiency of vitamin B2 in female non-aboriginals (48%), the lack of vitamin E in men (22%) and vitamin D and BC in 70-78% of the surveyed dictate the need for targeted enrichment of the diet of people living in extreme conditions of the Russian Arctic with multivitamin complexes.