Corrigendum: Histological Correlates of Diffusion-Weighted Magnetic Resonance Microscopy in a Mouse Model of Mesial Temporal Lobe Epilepsy.

[This corrects the article DOI: 10.3389/fnins.2020.00543.].

Histological Correlates of Diffusion-Weighted Magnetic Resonance Microscopy in a Mouse Model of Mesial Temporal Lobe Epilepsy.

Mesial temporal lobe epilepsy (MTLE) is the most common type of focal epilepsy. It is frequently associated with abnormal MRI findings, which are caused by underlying cellular, structural, and chemical changes at the micro-scale. In the current study, it is investigated to which extent these alterations correspond to imaging features detected by high resolution magnetic resonance imaging in the intrahippocampal kainate mouse model of MTLE. Fixed hippocampal and whole-brain sections of mouse brain tissue from nine animals under physiological and chronically epileptic conditions were examined using structural and diffusion-weighted MRI. Microstructural details were investigated based on a direct comparison with immunohistochemical analyses of the same specimen. Within the hippocampal formation, diffusion streamlines could be visualized corresponding to dendrites of CA1 pyramidal cells and granule cells, as well as mossy fibers and Schaffer collaterals. Statistically significant changes in diffusivities, fractional anisotropy, and diffusion orientations could be detected in tissue samples from chronically epileptic animals compared to healthy controls, corresponding to microstructural alterations (degeneration of pyramidal cells, dispersion of the granule cell layer, and sprouting of mossy fibers). The diffusion parameters were significantly correlated with histologically determined cell densities. These findings demonstrate that high-resolution diffusion-weighted MRI can resolve subtle microstructural changes in epileptic hippocampal tissue corresponding to histopathological features in MTLE.

Microscale 3D imaging by magnetic resonance force microscopy using full-volume Fourier- and Hadamard-encoding.

Three-dimensional spatially resolved full-volume imaging by magnetic resonance force microscopy at room temperature is described. Spatial resolution in z-dimension is achieved by using the magnetic-field gradient of a ferromagnetic particle that is also used for the force detection of the magnetic resonance. The gradient of the radiofrequency pulses generated by two separate wire-bonded microcoils is used for spatial resolution in x- and y-dimension. To enhance the sensitivity of our measurement Hadamard- and Fourier-encoding schemes are applied due to their multiplex effect. Measurements were taken on a patterned (NH4)2SO4 crystal sample. From the calculated magnetic field distributions, a 3D image was reconstructed with a voxel volume of about 5 µm3 (1.2 µm × 3.0 µm × 1.4 µm in x-, y- and z-dimension).

Relevance of the Implementation of Teeth in Three-Dimensional Vocal Tract Models.

Purpose: Recently, efforts have been made to investigate the vocal tract using magnetic resonance imaging (MRI). Due to technical limitations, teeth were omitted in many previous studies on vocal tract acoustics. However, the knowledge of how teeth influence vocal tract acoustics might be important in order to estimate the necessity of implementing teeth in vocal tract models. The aim of this study was therefore to estimate the effect of teeth on vocal tract acoustics. Method: The acoustic properties of 18 solid (3-dimensional printed) vocal tract models without teeth were compared to the same 18 models including teeth in terms of resonance frequencies (fRn). The fRn were obtained from the transfer functions of these models excited by white noise at the glottis level. The models were derived from MRI data of 2 trained singers performing 3 different vowel conditions (/i/, /a/, and /u/) in speech and low-pitched and high-pitched singing. Results: Depending on the oral configuration, models exhibiting side cavities or side branches were characterized by major changes in the transfer function when teeth were implemented via the introduction of pole-zero pairs. Conclusions: To avoid errors in modeling, teeth should be included in 3-dimensional vocal tract models for acoustic evaluation. Supplemental Material: https://doi.org/10.23641/asha.5386771.

A comparison of Lenz lenses and LC resonators for NMR signal enhancement.

High signal-to-noise ratio (SNR) of the NMR signal has always been a key target that drives massive research effort in many fields. Among several parameters, a high filling factor of the MR coil has proven to boost the SNR. In case of small-volume samples, a high filling factor and thus a high SNR can be achieved through miniaturizing the MR coil. However, under certain circumstances, this can be impractical. In this paper, we present an extensive theoretical and experimental investigation of the inductively coupled LC resonator and the magnetic Lenz lens as two candidate approaches that can enhance the SNR in such circumstances. The results demonstrate that the narrow-band LC resonator is superior in terms of SNR, while the non-tuned nature of the Lenz lens makes it preferable in broadband applications.

A Comparison of Different Methods to Generate Tooth Surface Models Without Applying Ionizing Radiation for Digital 3-Dimensional Image Fusion With Magnetic Resonance Imaging-Based Data of the Head and Neck Region.

OBJECTIVE: The 3-dimensional display of the vocal tract and teeth is necessary in numerous clinical and scientific contexts. Due to the different tissue properties this can only be achieved by combining different imaging techniques. A comparison of methods to record the tooth surface to create combined models of the vocal tract and teeth without applying ionizing radiation is displayed in this study. METHODS: Four methods to record the tooth surface were compared concerning their clinical accuracy after combination with magnetic resonance imaging (MRI)-based vocal tract models. With 2 of the presented methods the information of the tooth surface was obtained from MRI data. With the other 2 methods, the tooth surface was captured using a digital and a conventional impression technique. RESULTS: Impression techniques received the best rating results. CONCLUSIONS: The digital 3-dimensional image fusion of dental impression and MRI resulted in a virtual model of the vocal tract and teeth with a high clinical accuracy without applying ionizing radiation.

Articulation and vocal tract acoustics at soprano subject's high fundamental frequencies.

The role of the vocal tract for phonation at very high soprano fundamental frequencies (F0s) is not yet understood in detail. In this investigation, two experiments were carried out with a single professional high soprano subject. First, using two dimensional (2D) dynamic real-time magnetic resonance imaging (MRI) (24 fps) midsagittal and coronal vocal tract shapes were analyzed while the subject sang a scale from Bb5 (932 Hz) to G6 (1568 Hz). In a second experiment, volumetric vocal tract MRI data were recorded from sustained phonations (13 s) for the pitches C6 (1047 Hz) and G6 (1568 Hz). Formant frequencies were measured in physical models created by 3D printing, and calculated from area functions obtained from the 3D vocal tract shapes. The data showed that there were only minor modifications of the vocal tract shape. These changes involved a decrease of the piriform sinus as well as small changes of tongue position. Formant frequencies did not exhibit major differences between C6 and G6 for F1 and F3, respectively. Only F2 was slightly raised for G6. For G6, however, F2 is not excited by any voice source partial. Therefore, this investigation was not able to confirm that the analyzed professional soprano subject adjusted formants to voice source partials for the analyzed F0s.

Optical characterization of adaptive fluidic silicone-membrane lenses.

We present an extended optical characterization of an adaptive microfluidic silicone-membrane lens at a wavelength of 633 nm, respectively 660 nm. Two different membrane variations; one with a homogeneous membrane thickness, and one with a shaped cross section, have been realized. This paper includes the theoretical predictions of the optical performance via FEM simulation and ray tracing, and a subsequent orientation dependent experimental analysis of the lens quality which is measured with an MTF setup and a Mach-Zehnder interferometer. The influence of the fabrication process on the optical performance is also characterized by the membrane deformation in the non-deflected state. The lens with the homogeneous membrane of 5 mm in diameter and an aperture of 2.5 mm indicates an almost orientation independent image quality of 117 linepairs/mm at a contrast of 50%. The shaped membrane lenses show a minimum wave front error of WFE(RMS) = 24 nm, and the lenses with a planar membrane of WFE(RMS) = 31 nm at an aperture of 2.125 mm.