ABSTRACT
Spontaneous and initiated by hydrogen peroxide chemiluminescence was evaluated before and after freezing of the CSF from 20 patients with various spinal tumors and 10 controls with intervertebral lumbar osteochondrosis. The intensity of the above chemiluminescence was found significantly different in tumors and osteochondrosis. Cryotreatment induced marked changes in CSF chemiluminescence, especially in extramedullary spinal tumors. CSF treatment with hydrogen peroxide entailed alterations in free radical oxidation which can serve an additional criterion in combined diagnosis of spinal cord tumors.
Subject(s)
Peroxides/cerebrospinal fluid , Spinal Cord Neoplasms/cerebrospinal fluid , Cerebrospinal Fluid/drug effects , Free Radicals , Freezing , Humans , Hydrogen Peroxide/pharmacology , Lumbar Vertebrae , Luminescent Measurements , Osteochondritis/cerebrospinal fluid , Spondylitis/cerebrospinal fluidABSTRACT
The article discusses a new method for recognising tumors of the spinal cord--spontaneous and photoinduced chemoluminescence of the cerebrospinal fluid. Twenty-seven patients with tumors of the spinal cord of various localization and histological structure and 15 individuals of the control group were examined. Statistically significant differences in chemoluminescence in extra- and intramedullary tumors and in the control group were revealed. The effect of intensified luminescence of the cerebrospinal fluid in exposure to ultraviolet radiation was demonstrated, especially in cases with extramedullary tumors. The described method is an auxiliary criterion in c.s.f. diagnosis of spinal tumors.
Subject(s)
Luminescent Measurements , Spinal Cord Neoplasms/cerebrospinal fluid , Biophysical Phenomena , Biophysics , Cerebrospinal Fluid/radiation effects , Humans , Lumbar Vertebrae , Mathematics , Osteochondritis/cerebrospinal fluid , Spinal Cord Neoplasms/diagnosis , Ultraviolet RaysABSTRACT
Hydrogen peroxide-induced chemiluminescence of red cells of various age groups in diabetics was under study. A clinical, laboratory, and instrumental examination of 15 patients with diabetes mellitus was carried out. Red cell fraction chemiluminescence was examined in quantum metric equipment with FEU-39A and FEU-140 radiation detectors sensitive to the visible and UV regions. Intensive fluorescence of the third and, in some cases, of the fourth red cell fractions was observed in the diabetics, in contrast to the reference group of normal subjects (more than 3-fold higher in the patients). A high level of peroxide chemiluminescence observed at the end of examinations of the third and fourth fractions of the diabetics' red cells is an evidence of a high concentration of lipid peroxidation products in the diabetics, increased vs. the norm. Diabetes mellitus involves activation of the red cell fraction free-radical processes, leading to a preterm destruction of functionally active red cells first of all.
Subject(s)
Diabetes Mellitus, Type 1/blood , Erythrocyte Aging , Adult , Free Radicals , Humans , Lipid Peroxidation , Luminescent Measurements , MaleSubject(s)
Erythrocytes/cytology , Osmotic Fragility , Adult , Cell Separation , Female , Humans , Pre-Eclampsia/blood , PregnancySubject(s)
Antitubercular Agents/adverse effects , Drug Hypersensitivity/diagnosis , Tuberculosis, Pulmonary/drug therapy , Adult , Antitubercular Agents/blood , Antitubercular Agents/therapeutic use , Drug Hypersensitivity/etiology , Female , Humans , Luminescent Measurements , Male , Middle Aged , Thermal Conductivity , Tuberculosis, Pulmonary/bloodABSTRACT
The microthermal method of red blood cells' division according to their age is based on the formation of a temperature gradient with the aid of microthermoresistor. The red blood cells of different age fractions are consecutively localized and selected in the zone of microthermoresistor. They differ from one another according to their biochemical indices. The microthermal method of erythrocytes' division offers a possibility of differential studying the blood cells.
