Dragon fruit farming by-products as an important source of several glycosylated flavonoids.

Plants are globally used as an alternative for conventional drugs in treatment of many diseases. A significant proportion of medicinal properties of plants is addressed to antioxidant constituents as flavonoids and phenolic acids. The genus Selenicereus, known as dragon fruit, has about 15 species of epiphytic or hemiepiphytic cactus with hotspots in the tropical region of Mexico. Recently, these plants have been the focus of pharmacological studies due to the antioxidant activity of its fruits. Although many studies have investigated the biological activities of fruits, few studies investigated the chemical constituents and biological activities of cladodes. Despite the little knowledge about cladodes, it has been already observed to have higher antioxidant activities and other biological activities than the fruits. Besides this, cladodes are by-products resulting from the year-round pruning. Another important point about studies involving dragon fruit is that none of them identify the variety that is being used. As it is a commercial plant, it has many varieties created by artificial selection and hybridization such as many other food plants. In this study we found that varieties from the same species showed quantitative and qualitative differences in the metabolite profile using LC-MS. Metabolite profile from Cerrado (Selenicereus setaceus) was closer to Branca (Selenicereus undatus) than Thick King (S. setaceus), as well as Golden (S. undatus) was closer to Thick King than Branca. These results show that it is essential to identify varieties that are being used in the studies, whereas studies that attempt to replicate the experiments or use these plants for phytopreparations are prone to mislead.

Fast and green universal method to analyze and quantify anthocyanins in natural products by UPLC-PDA.

This work developed a universal UPLC-PDA method based on safe reagents to analyze anthocyanins from different foods. Nine foods were studied by the developed chromatographic method, which was constructed using a solid core C18 column and a binary mobile phase composed of (A) water (0.25 molcitric acid.Lsolvent-1), and (B) ethanol. A total running time of 6 min was obtained, the faster comprehensive method for anthocyanins analysis. Mass spectrometry analysis was employed to identify a comprehensive set of 53 anthocyanins comprising glycosylated and acylated cyanidin, pelargonidin, malvidin, peonidin, petunidin, and delphinidin derivatives. Cyanidin-3-O-glucoside (m/z+ 449) and cyanidin-3-O-rutinoside (m/z+ 595) were used as standards to validate the accuracy of the developed method. The analytical parameters were evaluated, including intra-day and inter-day precision, robustness, repeatability, retention factor (k), resolution, and peak symmetry factor. The current method demonstrated excellent chromatographic resolution, making it a powerful tool for analyzing anthocyanins pigments.

Ultra-high-performance liquid chromatography using a fused-core particle column for fast analysis of propolis phenolic compounds.

Propolis is a bee product with a complex chemical composition formed by several species from different geographical origins. The complex propolis composition requires an accurate and reproducible characterization of samples to standardize the quality of the material sold to consumers. This work developed an ultra-high-performance liquid chromatography with a photodiode array detector method to analyze propolis phenolic compounds based on the two key propolis biomarkers, Artepillin C and p-Coumaric acid. This choice was made due to the complexity of the sample with the presence of several compounds. The optimized method was hyphenated with mass spectrometry detection allowing the detection of 23 different compounds. A step-by-step strategy was used to optimize temperature, flow rate, mobile phase composition, and re-equilibration time. Reverse-phase separation was achieved with a C18 fused-core column packed with the commercially available smallest particles (1.3 nm). Using a fused-core column with ultra-high-performance liquid chromatography allows highly efficient, sensitive, accurate, and reproducible determination of compounds extracted from propolis with an outstanding sample throughput and resolution. Optimized conditions permitted the separation of the compounds in 5.50 min with a total analysis time (sample-to-sample) of 6.50 min.