Evaluation of the Effectiveness of the National Clean Air Programme in Terms of Health Impacts from Exposure to PM2.5 and NO2 Concentrations in Poland.

The health impact of air quality has recently become an emerging issue. Many regions, especially densely populated regions, have deteriorating air quality. The primary source of pollution in Poland is the municipal sector. Air pollutants have a negative impact on human health, contribute to premature deaths, and are the cause of various diseases. Over recent years, Europe's air quality has largely improved due to several measures to reduce pollutant emissions. The following paper presents the impact of annual average PM2.5 and NO2 concentrations on premature deaths in Poland in 2019. Exposure to PM2.5 caused more than 19 000 premature deaths, and exposure to NO2 contributed to around 6000 premature deaths in 2019. Taking 2019 as a baseline, the impact of the envisaged implementation of the national Clean Air Programme on the number of premature deaths is analysed. Implementing the mitigation measures focused on replacing inefficient stoves and boilers in individual households would significantly improve air quality, mainly particulate matter. Reducing PM2.5 concentrations would reduce premature deaths by around 3000 cases, while for NO2, the number of premature deaths would not change much.

Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter.

Global dust storms on Mars are rare1,2 but can affect the Martian atmosphere for several months. They can cause changes in atmospheric dynamics and inflation of the atmosphere3, primarily owing to solar heating of the dust3. In turn, changes in atmospheric dynamics can affect the distribution of atmospheric water vapour, with potential implications for the atmospheric photochemistry and climate on Mars4. Recent observations of the water vapour abundance in the Martian atmosphere during dust storm conditions revealed a high-altitude increase in atmospheric water vapour that was more pronounced at high northern latitudes5,6, as well as a decrease in the water column at low latitudes7,8. Here we present concurrent, high-resolution measurements of dust, water and semiheavy water (HDO) at the onset of a global dust storm, obtained by the NOMAD and ACS instruments onboard the ExoMars Trace Gas Orbiter. We report the vertical distribution of the HDO/H2O ratio (D/H) from the planetary boundary layer up to an altitude of 80 kilometres. Our findings suggest that before the onset of the dust storm, HDO abundances were reduced to levels below detectability at altitudes above 40 kilometres. This decrease in HDO coincided with the presence of water-ice clouds. During the storm, an increase in the abundance of H2O and HDO was observed at altitudes between 40 and 80 kilometres. We propose that these increased abundances may be the result of warmer temperatures during the dust storm causing stronger atmospheric circulation and preventing ice cloud formation, which may confine water vapour to lower altitudes through gravitational fall and subsequent sublimation of ice crystals3. The observed changes in H2O and HDO abundance occurred within a few days during the development of the dust storm, suggesting a fast impact of dust storms on the Martian atmosphere.

Publisher Correction: Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter.

The surname of author Cathy Quantin-Nataf was misspelled 'Quantin-Nata' , authors Ehouarn Millour and Roland Young were missing from the ACS Science Team list, and minor changes have been made to the author and affiliation lists; see accompanying Amendment. These errors have been corrected online.

A multicommutated tester of bioreactors for flow analysis.

Enzymes are often used in the modern analytical procedures allowing selective recognition and conversion of target analytes into easily detected products. In flow analysis systems, enzymes are predominantly applied in the immobilized forms as flow-through bioreactors. In this research the multicommutated flow analysis (MCFA) system for evaluation and comparison of analytical parameters of bioreactors has been developed. The MCFA manifold allows simultaneous testing up to four bioreactors, but if necessary their number can be easily increased. The system allows comparison of several parameters of tested bioreactors including activity, repeatability, reproducibility, operational and storage stability. The performance of developed bioreactor tester is presented using urea-urease model system based on plastic open-tubular bioreactor with covalently immobilized enzyme. Product of enzymatic reaction is detected using two different chemical methods and by dedicated optoelectronic ammonium detectors. Moreover, the utility of developed MCFA manifold for evaluation of other enzyme bioreactors is demonstrated.

Synthesis and pharmacological properties of new GABA uptake inhibitors.

BACKGROUND: γ-Aminobutanoic acid (GABA) is the principal inhibitory neurotransmitter in the mammalian central nervous system. The identification and subsequent development of the GABA transport inhibitors which enhance the GABA-ergic transmission has shown the important role that GABA transporters play in the control of numerous functions of the nervous system. Compounds which inhibit GABA uptake are used as antiepileptic drugs (tiagabine - a selective GAT1 inhibitor), they are also being investigated for other indications, including treatment of psychosis, general anxiety, sleep disorders, drug addiction or acute and chronic pain. METHODS: In this paper, the synthesis of 2-substituted-4-(1,3-dioxoisoindolin-2-ylo)-butanamides and 2-substituted-4-aminobutanoic acids derivatives is described. These compounds were tested in vitro for their ability to inhibit GABA uptake. The inhibitory potency towards murine plasma membrane GABA transporters (mGAT1-4) was performed as [(3)H]GABA uptake assay based on stably transfected HEK cells. Compound 18, which demonstrated the highest affinity for mGAT1-4 (pIC(50) ranged from 4.42 for mGAT1 to 5.07 for mGAT3), was additionally investigated in several behavioral tests in mice. RESULTS: Compound 18 increased the locomotor activity (14-38%) and had anxiolytic-like properties in the four-plate test (ED(50) = 9.3 mg/kg). It did not show analgesic activity in acute pain model, namely the hot plate test, however, it was antinociceptive in the acetic acid-induced writhing test (ED(50) = 15.3 mg/kg) and in the formalin model of tonic pain. In the latter assay, it diminished nocifensive behavior in both phases and in the first (neurogenic) phase of this test the obtained ED(50) value (5.3 mg/kg) was similar to morphine (3.0 mg/kg). CONCLUSION: Compound 18 exhibited significant anxiolytic-like properties and was antinociceptive in some models of pain in mice. Moreover, it did not impair animals' motor coordination in the chimney test. Some of the described pharmacological activities of compound 18 can be partly explained based on its affinity for plasma membrane GABA transporters.

[Analysis of nutritional status disorders in patients with chronic obstructive pulmonary disease]. / Analiza zaburzen stanu odzywienia u pacjentów z przewlekla obturacyjna choroba pluc.

INTRODUCTION: Among the most common extrapulmonary manifestations of COPD are nutritional status disorders. The specific loss of weight, called cachexia, characterized by loss of lean body mass in some COPD patients is observed. The aim of the study was the quantitative and qualitative analysis of COPD patients' nutritional status disturbances. MATERIAL AND METHODS: Fifty-five patients in different stages of COPD--43 males and 12 females (mean age 62.31 +/- 11.08) and 32 subjects from a control group (mean age 57.43 +/- 8.79) participated in the study. In both groups nutritional status was assessed using different indicators such as PIBW--percentage of ideal body weight, BMI--body mass index, FFMI--fat-free mass index and FMI--fat mass index. RESULTS: Malnutrition measured by PIBW, BMI, BMI percentiles, and FFMI was observed in 5.45%, 3.64%, 3.64% and 18.18% of COPD patients, respectively, and in the control group 3.12%, 0%, 3.12% and 3.12%, respectively. The BMI mean value did not differ significantly between groups. It was confirmed that cachexia assessed by FFMI occured more frequently in COPD patients than in the control group--19.05 kg/m2 vs. 20,55 kg/m2 (p = 0.04). CONCLUSIONS: 1. Nutritional status disorders pose a serious problem, which concerns about 1/5 of the COPD population. 2. It is necessary to perform quantitative analysis of nutritional status (assessment of lean and fat mass) because indicators of body mass (PIBW, BMI) are not sufficient for cachexia detection. 3. Having normal body mass does not exclude the possibility of nutritional status disorders in COPD patients.

Evaluating a Canadian regional air quality model using ground-based observations in north-eastern Canada and United States.

The simulated concentrations from a numerical 3-dimensional regional air quality model (MC2AQ) are compared to those of ground-based observations in north-eastern Canada and the United States. The model has oxidant chemistry for both inorganic and organic species and deposition routines driven online by a mesoscale compressible community meteorological model (MC2). A standard emission inventory of anthropogenic, natural and biogenic sources for the year 1990 for 21 atmospheric trace species was used in the simulation. The model was run for July 1999, because of the occurrence of a high ozone episode and the availability of the monitoring data for surface O3, SO2, NO, NO2 and NOx. The comparisons during the episode show that the model performs quite well for predicting concentrations and diurnal variations of the surface ozone. The predictions for other gaseous species show some discrepancies with observations, but they are consistent with the results from other models evaluated in the literature. The uncertainties in the emission inventory for these species might be the main causes of the discrepancies. Further studies are needed to improve the predictability of SO and NOx, especially as the model is developed to include particulate matter formation as a result of these gaseous precursors.