Antiviral activities of Cholistani plants against common poultry viruses.

Herbal medicines are becoming more popular and acceptable day by day due to their effectiveness, limited side effects, and cost-effectiveness. Cholistani plants are reported as a rich source of antibacterial, antifungal, antiprotozoal, antioxidant, and anticancer agents. The current study has evaluated antiviral potential of selected Cholistani plants. The whole plants were collected, ground and used in extract formation with n-hexane, ethyl acetate and n-butanol. All the extracts were concentrated by using a rotary evaporator and concentrate was finally dissolved in an appropriate vol of the same solvent. All of the extracts were tested for their antiviral potential by using 9-11 days old chick embryonated eggs. Each extract was tested against the Avian Influenza virus H9N2 strain (AIV), New Castle Disease virus Lasoota strain (NDV), Infectious bronchitis virus (IBV) and an Infectious bursal disease virus (IBDV). Hemagglutination test (HA) and Indirect Hemagglutination (IHA) tests were performed for different viruses. The overall order of the antiviral potential of Cholistani plants against viruses was NDV>IBV>IBDV>AIV. In terms of antiviral activity from extracts, the order of activity was n-butanol>ethyl acetate>n-hexane. The medicinal plants Achyranthes aspera, Neuroda procumbens, Panicum antidotale, Ochthochloa compressa and Suaeda fruticose were very effective against all four poultry viruses through their extracts. The low IC50 values of these extracts confirm the high antiviral potential against these viruses. It is worth to mention that Achyranthes aspera was found positive against IBDV through all its extracts which overcome the problem of unavailability of any known drug against IBDV. In short, the study proved that Cholistani plants are rich source of antiviral agent and their extracts can be used as good source of antiviral drugs both in crude and in purified form.

Antiviral activities of Cholistani plants against common poultry viruses

@#Herbal medicines are becoming more popular and acceptable day by day due to their effectiveness, limited side effects, and cost-effectiveness. Cholistani plants are reported as a rich source of antibacterial, antifungal, antiprotozoal, antioxidant, and anticancer agents. The current study has evaluated antiviral potential of selected Cholistani plants. The whole plants were collected, ground and used in extract formation with n-hexane, ethyl acetate and n-butanol. All the extracts were concentrated by using a rotary evaporator and concentrate was finally dissolved in an appropriate vol of the same solvent. All of the extracts were tested for their antiviral potential by using 9-11 days old chick embryonated eggs. Each extract was tested against the Avian Influenza virus H9N2 strain (AIV), New Castle Disease virus Lasoota strain (NDV), Infectious bronchitis virus (IBV) and an Infectious bursal disease virus (IBDV). Hemagglutination test (HA) and Indirect Hemagglutination (IHA) tests were performed for different viruses. The overall order of the antiviral potential of Cholistani plants against viruses was NDV>IBV>IBDV>AIV. In terms of antiviral activity from extracts, the order of activity was n-butanol>ethyl acetate>n-hexane. The medicinal plants Achyranthes aspera, Neuroda procumbens, Panicum antidotale, Ochthochloa compressa and Suaeda fruticose were very effective against all four poultry viruses through their extracts. The low IC50 values of these extracts confirm the high antiviral potential against these viruses. It is worth to mention that Achyranthes aspera was found positive against IBDV through all its extracts which overcome the problem of unavailability of any known drug against IBDV. In short, the study proved that Cholistani plants are rich source of antiviral agent and their extracts can be used as good source of antiviral drugs both in crude and in purified form.

Comparison of Multilevel Cervical Disc Replacement and Multilevel Anterior Discectomy and Fusion: A Systematic Review of Biomechanical and Clinical Evidence.

OBJECTIVE: The aim of this study was to comprehensively compare the clinical and biomechanical efficiency of anterior cervical discectomy and fusion (ACDF) with anterior cervical disc replacement (ACDR) for treatment of multilevel cervical disc disease using a meta-analysis and systematical review. METHODS: A literature search was performed using PubMed, MEDLINE, EMBASE, and the Cochrane Library for articles published between January 1960 and December 2017. Both clinical and biomechanical parameters were analyzed. Statistical tests were conducted by Revman 5.3. Nineteen studies including 10 clinical studies and 9 biomechanical studies were filtered out. RESULTS: The pooled results for clinical efficiency showed that no significant difference was observed in blood loss (P = 0.09; mean difference [MD], 7.38; confidence interval [CI], -1.16 to 15.91), hospital stay (P = 0.33; MD, -0.25; CI, -0.76 to 0.26), Japanese Orthopaedic Association scores (P = 0.63; MD, -0.11; CI, -0.57 to 0.34), visual analog scale (P = 0.08; MD, -0.50; CI, -1.06 to 0.05), and Neck Disability Index (P = 0.33; MD, -0.55; CI, -1.65 to 0.56) between the 2 groups. Compared with ACDF, ACDR did show increased surgical time (P = 0.03; MD, 31.42; CI, 2.71-60.14). On the other hand, ACDR showed increased index range of motion (ROM) (P < 0.00001; MD, 13.83; CI, 9.28-18.39), lower rates of adjacent segment disease (ASD) (P = 0.001; odds ratio [OR], 0.27; CI, 0.13-0.59), complications (P = 0.006; OR, 0.62; CI, 0.45-0.87), and rate of subsequent surgery (P < 0.00001; OR, 0.25; CI, 0.14-0.44). As for biomechanical performance, ACDR maintained index ROM and avoided compensation in adjacent ROM and tissue pressure. CONCLUSIONS: Multilevel ACDR may be an effective and safe alternative to ACDF in terms of clinical and biomechanical performance. However, further multicenter and prospective studies should be conducted to obtain a stronger and more reliable conclusion.

Effects of Varying Levels of Fungal (Arachniotus sp.) Treated Wheat Straw as an Ingredient of Total Mixed Ration on Growth Performance and Nutrient Digestibility in Nili Ravi Buffalo Calves.

The study was carried out to explore the effects of replacing wheat straw with fungal treated wheat straw as an ingredient of total mixed ration (TMR) on the growth performance and nutrient digestibility in Nili Ravi buffalo male calves. Fungal treated wheat straw was prepared using Arachniotus sp. Four TMRs were formulated where wheat straw was replaced with 0 (TMR1), 33 (TMR2), 67 (TMR3), and 100% (TMR4) fungal treated wheat straw in TMR. All TMRs were iso-caloric and iso-nitrogenous. The experimental TMRs were randomly assigned to four groups of male calves (n = 6) according to completely randomized design and the experiment continued for four months. The calves fed TMR2 exhibited a significant improve in dry matter intake, average daily weight gain, feed conversion ratio and feed economics compared to other groups. The same group also showed higher digestibility of dry matter, crude protein, neutral-, and acid detergent fibers than those fed on other TMRs. It is concluded that TMR with 33% fungal-treated wheat straw replacement has a potential to give an enhanced growth performance and nutrient digestibility in male Nili Ravi buffalo calves.

Role of selenium in male reproduction - a review.

The role of Se and various selenoproteins in male reproductive performance is reviewed. Development of male reproductive tissue requires an optimal level of Se in testis, and a small deviation, either deficiency or excess, leads to abnormal development. Selenium is a constituent of selenoproteins including GPx1, GPx3, mGPx4, cGPx4, and GPx5 that protect against oxidative damage to spermatozoa throughout the process of sperm maturation, whereas selenoproteins, such as mGPx4 and snGPx4, serve as structural components of mature spermatozoa. Thus Se and selenoproteins ensure viability of spermatozoa as well as providing protection against reactive oxygen species. Gene knock-out studies of selenoproteins revealed that their absence during spermatogenesis results in abnormal spermatozoa, which in turn affects semen quality and fertility. Deviation from the optimal quantities of dietary Se, both above or below, may cause multiple abnormalities of spermatozoa and affect motility and fertility. Libido may also be increased by Se. Dietary Se should be in optimal quantity to maintain reproductive function in males and to avoid infertility.

The Objective Structured Clinical Examination (OSCE): AMEE Guide No. 81. Part II: organisation & administration.

The organisation, administration and running of a successful OSCE programme need considerable knowledge, experience and planning. Different teams looking after various aspects of OSCE need to work collaboratively for an effective question bank development, examiner training and standardised patients' training. Quality assurance is an ongoing process taking place throughout the OSCE cycle. In order for the OSCE to generate reliable results it is essential to pay attention to each and every element of quality assurance, as poorly standardised patients, untrained examiners, poor quality questions and inappropriate scoring rubrics each will affect the reliability of the OSCE. The validity will also be influenced if the questions are not realistic and mapped against the learning outcomes of the teaching programme. This part of the Guide addresses all these important issues in order to help the reader setup and quality assure their new or existing OSCE programmes.

The Objective Structured Clinical Examination (OSCE): AMEE Guide No. 81. Part I: an historical and theoretical perspective.

The Objective Structured Clinical Examination (OSCE) was first described by Harden in 1975 as an alternative to the existing methods of assessing clinical performance (Harden et al. 1975). The OSCE was designed to improve the validity and reliability of assessment of performance, which was previously assessed using the long case and short case examinations. Since then the use of the OSCE has become widespread within both undergraduate and postgraduate clinical education. We recognise that the introduction of the OSCE into an existing assessment programme is a challenging process requiring a considerable amount of theoretical and practical knowledge. The two parts of this Guide are designed to assist all those who intend implementing the OSCE into their assessment systems. Part I addresses the theoretical aspects of the OSCE, exploring its historical development, its place within the range of assessment tools and its core applications. Part II offers more practical information on the process of implementing an OSCE, including guidance on developing OSCE stations, choosing scoring rubrics, training examiners and standardised patients and managing quality assurance processes. Together we hope these two parts will act as a useful resource both for those choosing to implement the OSCE for the first time and also those wishing to quality assure their existing OSCE programme.

Influence of varying dietary electrolyte balance on broiler performance under tropical summer conditions.

A 6-week feeding trial was conducted to determine the effects of varying dietary electrolyte balance (DEB) on growth performance and physiological responses in broiler chicks reared during hot summer months (26.1-37.5 degrees C). The average minimum and maximum room temperatures recorded from 14 to 42 days of age were 26.1 and 37.5 degrees C, respectively, with relative humidity ranging from 51% to 55%. Five DEB treatments supplying 0, 50, 150, 250 and 350 (Na(+)+K(+)-Cl(-)) mEq/kg of diet were prepared by adding NaHCO(3) and/or NH(4)Cl to basal diet that contained 185 mEq/kg from 1 to 28 days and 172 mEq/kg from 29 to 42 days. These DEB diets were randomly offered to six experimental units of 20 chicks each. Improved 42-day body weight gain (BWG), feed:gain (F:G) and reduced mortality was noted with DEB 50, 150 and 250 mEq/kg compared to DEB 0 and 350 mEq/kg. A quadratic effect of increasing DEB level was observed on BWG and F:G, whereas the effect was linear for feed intake, water intake and litter moisture. The DEB 50, 150 and 250 maintained better blood pH, pCO(2) and HCO(3) concentrations during acute heat stress periods than that of 0 and 350. The blood heterophil and lymphocyte percent as well as heterophil:lymphocyte were remained unaffected. The lowest DEB (0 mEq/kg) diet reduced the feed intake and promoted metabolic acidosis. At 350 mEq/kg DEB, water intake and metabolic alkalosis were promoted probably due to high levels of dietary NaHCO(3). The study concluded that overall better performance was recorded with DEB 50, 150 and 250 mEq/kg. These results indicated that single optimal DEB value could not be recommended to combat heat stress in broilers.

Effect of low-protein diets having constant energy-to-protein ratio on performance and carcass characteristics of broiler chickens from one to thirty-five days of age.

A trial was conducted to determine the effect of low-protein diets with constant ME:CP ratio on performance and carcass characteristics of broilers from 1 to 35 d of age. Four experimental diets were formulated to have 4 levels of CP and ME, respectively, in each phase: 23, 22, 21, and 20% CP with 3,036, 2,904, 2,772, and 2,640 kcal/kg in the starter phase (1 to 10 d); 22, 21, 20, and 19% CP with 3,146, 3,003, 2,860, and 2,717 kcal/kg in the grower phase (11 to 26 d); and 20, 19, 18, and 17% CP with 3,100, 2,945, 2,790, and 2,635 kcal/kg in the finisher phase (27 to 35 d). Digestible Lys was maintained at 1.10, 1.02, and 0.90% of the diet in the starter, grower, and finisher periods, respectively. A total of 1,760 one-day-old Hubbard broiler chickens were randomly divided into 16 experimental pens, 110 chickens in each pen, and each diet was offered to 4 replicates at random. Weight gain was linearly decreased (P < 0.001), whereas feed intake and feed conversion ratio were increased (P < 0.001) linearly as dietary protein and energy decreased during grower, finisher, and overall experimental periods. Protein efficiency ratio and energy efficiency ratio were decreased (P < 0.05) with low-CP and low-ME diets during the grower, finisher, and overall experimental period. However, carcass yield, breast meat yield, thigh yield, abdominal fat, and relative liver and heart weights were not affected by the treatments. Feeding broiler chickens low-CP diets with constant ME:CP ratio has adversely affected the growth performance, but carcass parameters were unaffected without any increase in abdominal fat content.

A national online survey of final year medical students' opinion on the General Medical Council's proposed reforms to the undergraduate medical assessment system.

BACKGROUND: The General Medical Council (GMC) is holding consultations in order to decide on the proposed changes to the undergraduate medical assessment. In the last round of consultation only eight medical students formally responded nationally. AIM: To determine the views of a larger proportion of final year medical students across the country on the proposed changes to the undergraduate medical assessment. METHOD: An online national survey of 10 medical schools, from which 401 responses from final year medical students were collected. RESULTS AND DISCUSSION: The results indicate the medical students' views on the GMC's proposed changes to standardise the assessment system. The majority of the students were in favour of having a say in any changes to their future assessment. They agreed with the principle that there should be a consistency between assessments at different medical schools and currently their results did not represent preparedness to practice.

Case report: managing a knotted Seldinger wire in the subclavian vein during central venous cannulation.

PURPOSE: To describe the successful removal of a knotted Seldinger wire from a subclavian vein, and review the design and structure of guidewires to formulate recommendations to minimize complications associated with the Seldinger technique. CLINICAL FEATURES: An 81-yr-old patient suffered from an intravascular knotting of a Seldinger wire during subclavian venous cannulation. We describe a technique for successful removal of knotted guidewire under fluoroscopic guidance using the vessel dilator of a central venous cannulation kit. In this case, the technique was successful without associated immediate or delayed complications. Although central venous cannulation with the Seldinger technique is a commonly performed procedure, it may result in numerous complications, including kinking, and rarely complete knotting of the guidewire. CONCLUSIONS: A thorough understanding of procedural complications and physical characteristics of the guidewire is vital in order to ensure patient safety when using the Seldinger technique for central venous cannulation. We have reviewed the relevant literature for guidewire design and structure, associated complications, and provide recommendations for safe use of guidewires.