ABSTRACT
We evaluated the cytostatic effect of the antihypoxic drug Oliphen (Polyoxiarilene) in comparison with the well known cytostatic and immunomodulating agent Ukrain (a semisynthetic compound derived from Chelidonium majus L. alkaloids). We used tumor cell lines M-HeLa and Hep-2/0-6-5, normal cell cultures from human embryo kidney and lung fibroblasts. The cytodestructive effect of Ukrain on both tumor cell lines was more significant at all tested concentrations. Oliphen was cytotoxic only against Hep-2 but not the HeLa cell line. The proliferative activity of both normal cell cultures decreased in the presence of Oliphen and Ukrain concentrations of 0.1 and 0.015% and was stable at 0.0015 and 0.00015%.
Subject(s)
Alkaloids/pharmacology , Antioxidants/pharmacology , Phenyl Ethers/pharmacology , Alkaloids/toxicity , Antioxidants/toxicity , Berberine Alkaloids , HeLa Cells , Humans , Phenanthridines , Phenyl Ethers/toxicity , Tumor Cells, CulturedABSTRACT
The complex pathogenetic therapy of severe (complicated) forms of influenza infection is proposed. The drugs with antioxidant, antiprotease or immunomodulating activities are used for the purpose. The clinical character of infection or stage of pathologic process are considered for appropriate drug application. The advantages of drugs with diversified mechanisms of action involved in the complex treatment of influenza are demonstrated.
Subject(s)
Antioxidants/therapeutic use , Influenza, Human/drug therapy , Protease Inhibitors/therapeutic use , Adult , Antioxidants/pharmacology , Drug Therapy, Combination , Female , Humans , Influenza, Human/immunology , Male , Middle Aged , Photochemotherapy , Protease Inhibitors/pharmacology , Severity of Illness Index , T-Lymphocytes/drug effectsABSTRACT
Acetylcholinesterase possessed a different activity in the membranes of young, mature and old human erythrocytes - it was greatest in the mature and least in the old cells. In the young and mature erythrocytes the enzyme existed in the form of 3- and in the old ones in the form of 2-molecular components. The data obtained suggested that the changes in the structural organization of acetylcholinesterase in the red cell membrane had a direct relationship to the aging of these cells.
Subject(s)
Acetylcholinesterase/blood , Erythrocyte Aging , Erythrocyte Membrane/enzymology , Erythrocytes/enzymology , Humans , Molecular Weight , Osmotic FragilityABSTRACT
Content of phosphoribosyl pyrophosphate and the activity of ribose phosphate pyrophosphokinase and AMP-pyrophosphorylase were studied in erythrocytes of healthy persons and of patients with various types of anemia. Deficiency of phosphoribosyl pyrophosphate and a decrease in the activity of enzymes studied were observed in erythrocytes under microspherocytic and hypoplastic anemia. The alterations correlated with impairments in energy metabolism. At the same time an activation of phosphoribosyl pyrophosphate enzymatic system and an increase of its content in erythrocytes did not depend on energy metabolism in Marchiafava--Micheli disease.
Subject(s)
Adenine Phosphoribosyltransferase/blood , Anemia/blood , Erythrocytes/metabolism , Pentosephosphates/blood , Pentosyltransferases/blood , Phosphoribosyl Pyrophosphate/blood , Phosphotransferases/blood , Ribose-Phosphate Pyrophosphokinase/blood , Adenosine Diphosphate/blood , Adenosine Triphosphatases/blood , Adenosine Triphosphate/blood , Anemia, Aplastic/blood , Energy Metabolism , Enzyme Activation , Glucosephosphate Dehydrogenase/blood , Hemoglobinuria, Paroxysmal/blood , Humans , Phosphogluconate Dehydrogenase/blood , Spherocytosis, Hereditary/bloodABSTRACT
Appearance of low molecular components of acetylcholinesterase from erythrocyte membranes was found by gel filtration on Sephadex G-200 and polyacrylamide gel disc electrophoresis. Content of these components was distinctly increased within ghe acute period of Marchiafava-Micheli disease.
Subject(s)
Acetylcholinesterase/blood , Anemia/enzymology , Erythrocytes/enzymology , Polycythemia Vera/enzymology , Anemia, Aplastic/enzymology , Anemia, Hemolytic, Autoimmune/enzymology , Cell Membrane/enzymology , Chromatography, Gel , Electrophoresis, Polyacrylamide Gel , Hemoglobinuria, Paroxysmal/enzymology , Humans , Molecular Weight , Spherocytosis, Hereditary/enzymologySubject(s)
Acetylcholinesterase/blood , Adenosine Triphosphatases/blood , Anemia/enzymology , Erythrocytes/enzymology , Anemia, Aplastic/enzymology , Anemia, Hemolytic, Autoimmune/enzymology , Hemoglobinuria, Paroxysmal/enzymology , Humans , Hyperbilirubinemia/enzymology , Leukemia, Lymphoid/enzymology , Leukemia, Myeloid/enzymology , Spherocytosis, Hereditary/enzymologyABSTRACT
Proteins of human erythrocyte membranes were studied in normal state and in different anaemias. After solubilization of cell "shadows" in phenol-urea-acetic acid-water system in membranes there were found, by means of polyacrylamide gel disc electrophoresis, 20 protein fractions with molecular weight from 48000 to 225000; seven of them glycoproteins. In 9 cases out of 36 a complete or partial disappearance of a glycoprotein component with molecular weight of 170000 was observed in erythrocyte membranes from patients with inherited spherocytosis and Markiafava--Mikely disease.
