ABSTRACT
The age dynamics of stable chromosome aberration (SCA) frequency was analysed by fluorescent in situ hybridization (FISH) in human blood lymphocytes derived from donors, irradiated by low doses of ionizing radiation (Chernobyl clean-up workers, nuclear weapon testers, etc.) and patients with hereditary premature aging--Werner's syndrome and Hutchinson-Gilford's syndrome. It was found that the level of SCA was age-dependent and increased in irradiated persons. So, the SCA level may be really an index of a so-called "radiation senescence", and may show a real biological age of irradiated persons. The patients with Werner's syndrome demonstrate increased SCA level in blood lymphocytes, corresponding to the premature aging of the organisms. But in the case of another form of premature aging--Hutchinson--Gilford's syndrome-- no rise of SCA level was found. Some possible reasons of such results are discussed.
Subject(s)
Aging/genetics , Chromosome Aberrations , Leukocytes, Mononuclear/radiation effects , Progeria/genetics , Werner Syndrome/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Aging/blood , Child , Child, Preschool , Chromosomes, Human, Pair 1/genetics , Chromosomes, Human, Pair 12/genetics , Chromosomes, Human, Pair 4/genetics , Chromosomes, Human, Pair 8/genetics , Humans , In Situ Hybridization, Fluorescence , Karyotyping , Middle Aged , Progeria/blood , Ukraine , Werner Syndrome/bloodABSTRACT
On peripheral lymphocytes of 5 cancer patients undergone wholebody therapeutic irradiation (at daily dose of 10 cGy up to total dose 50 cGy of 60Co gamma-rays) the dose response of unstable and stable chromosomal exchanges detected by FISH was compared with the dose response of the some aberrations in lymphocytes irradiated in vitro. The dose response fitted well to linear function. For dicentrics the lower slope of dose-response curve was found for in vivo irradiated lymphocytes as compared to the dose-response curve obtained for in vitro irradiated lymphocytes of the same patients. No difference between in vivo and in vitro irradiation of lymphocytes was found for translocations. The frequency of translocations increased faster with the dose than the frequency of dicentrics only in lymphocytes irradiated in vivo.
Subject(s)
Chromosome Aberrations , Gamma Rays , Lymphocytes/radiation effects , Adult , Aged , Bone Neoplasms/secondary , Cells, Cultured/radiation effects , Chi-Square Distribution , Chromosomes, Human, Pair 4/radiation effects , Chromosomes, Human, Pair 8/radiation effects , Cobalt Radioisotopes/therapeutic use , Cytogenetic Analysis , Data Interpretation, Statistical , Dose-Response Relationship, Radiation , Female , Humans , In Situ Hybridization, Fluorescence , Male , Middle Aged , Neoplasm Metastasis/radiotherapy , Neoplasms/genetics , Neoplasms/radiotherapy , Radiation Dosage , Radiotherapy Dosage , Translocation, Genetic , Whole-Body IrradiationABSTRACT
On peripheral lymphocytes of eight cancer patients undergone whole-body therapeutic irradiation (at daily dose of 10 cGy up to total dose of 50 cGy of 60Co gamma-rays) the dose-response of unstable chromosome exchanges (dicentrics and centric rings) was studied. This dose response fitted well linear function. The lower slope of dose-response curve was found for in vivo irradiated lymphocytes as compared to the dose response curve obtained for in vitro irradiated lymphocytes of the same patients. This finding seems to provide evidence that in case of protracted irradiation of individuals an absorbed dose could be underestimated if for biological dosimetry an in vitro dose response curve for unstable chromosome aberrations is used as referent one.
