Confocal laser scanning microscopy study of intercellular events in filopodia using 3-mercaptopropoinc acid capped CdSe/ZnS quantum dots.

Physico-chemical mobility of cells in three dimensions is dependent on the development of filipodia, which is the fundamental instinct for survival and other cellular functions in live cells. Specifically, our present research paper describes the synthesis of 3-Mercaptopropoinc acid (MPA) capped CdSe/ZnS quantum dots (QDs), which are biocompatible and utilized for cellular bioimaging applications. Using the pancreatic cell lines BXCP3 cells, we successfully demonstrated the applicability of MPA-capped QDs for intercellular filopodia imaging. Employing these QDs, we examined the dynamics of filopodia formation in real-time along the Z-axis by using confocal laser microscopy.

Effect of Silver Nanoparticles Against the Formation of Biofilm by Pseudomonas aeruginosa an In silico Approach.

Studies were undertaken to examine the mechanism of mediation of silver nanoparticles in inhibiting biofilm formation by Pseudomonas aeruginosa through LuxI/LuxR system of signal transduction. This study includes the basic signaling transduction mechanism LasR, QscR, RhlR, and Vfr signaling model systems. The arbitrary homology models built with the I-TASSER server were evaluated and validated with the Qmean web server. Based on the Z-score and the relative square mean distance (RMSD) values, the structures were validated. The interaction results of the nanoparticle with the rigid docking proved the requirement of minimal energy for the inhibition of the protein active site by the silver nanoparticle. This principle docking experiment suggests that the biofilm formation in Gram-negative bacteria can be inhibited by the silver nanoparticles at the signal transduction level. Graphical abstract Systematic outline of present study; Stage one provides the data sampling and generation of pdb systems to conform the structure of bacterial signal sytems like LasR/LasI; RhlR/RhrI; QscR/QscI; VfrR/VfrI. Stage two involves docking of silver nanoparticles with Bacterial signal protein strucutres which are listed in Stage one. The Final Stage involves in understanding the development of appropriate mechanism behind the biofilm inhibition by silver nanoparticles.