ABSTRACT
An enzyme immunoassay kit based on monoclonal antibodies (Mab) has been developed for measuring thyroid-stimulating hormone in human serum and plasma. Laboratory trials demonstrated its high sensitivity (3.1 microIU/ml), specificity, reliability, and accuracy. The analysis is simple and rapid (2.5 h), which is convenient for clinical use. The kit permits differentiation between normal subjects and patients with primary hypo- or hyperthyroidism and helps monitor the treatment efficacy. Comparison with the Amerlite TSH-60 kit, carried out on 357 sera, showed a high correlation coefficient (0.96) between the two kits.
Subject(s)
Immunoenzyme Techniques , Reagent Kits, Diagnostic , Thyrotropin/blood , Antibodies, Monoclonal , Diagnosis, Differential , Humans , Hyperthyroidism/diagnosis , Hypothyroidism/diagnosis , Sensitivity and SpecificityABSTRACT
The chromatographic behavior of the glycoprotein hormones from human pituitary glands and of placental origin [thyroid-stimulating hormone, luteinizing hormone and chorionic gonadotropin (CG)] was studied. It was shown that hydrophobic interaction chromatography on a microparticulate packing and anion-exchange HPLC can be applied for the purification of these hormones. Reversed-phase HPLC on wide-pore C4-bonded silica at neutral pH can be applied for the determination of the above hormones and for the isolation of pure CG and its subunits.
Subject(s)
Chorionic Gonadotropin/analysis , Chromatography, High Pressure Liquid/methods , Luteinizing Hormone/analysis , Thyrotropin/analysis , Chromatography, Ion Exchange , Humans , Hydrogen-Ion Concentration , Spectrophotometry, UltravioletABSTRACT
Somatogen, a human somatotropic hormone (HSH) prepared with the use of gene engineering (genHSH) does not differ from the highly purified substance of hypophysial hormone (hypHSH) in affinity to the antibodies produced on hypHSH and to the membrane receptor of the rabbit liver. 50 per cent inhibition of 125I hypHSH binding to the antibodies was observed at somatogen concentration of 8.2 ng/ml and hypHSH concentration of 7.9 ng/ml. The affinity constants of somatogen and hypHSH to the receptor amounted to 3.8 X 10(9) and 3.6 X 10(9) M-1, respectively. Somatogen for injections as a medicine contains 94.6 per cent of the hormone monomer and by the level of its homogeneity exceeds hypHSH as a medicine manufactured by Serano (Italy) and genHSH as a medicine manufactured by Kabi Vitrum (Sweden).
