ABSTRACT
Here, we describe amentoflavone-type biflavonoids, which were isolated from natural sources and were found to inhibit beta-secretase (BACE-1). The structure-activity relationship was studied, and compounds 1-8, 10, 17, and 18 showed BACE-1 inhibitory activity. Among these compounds, 2,3-dihydroamentoflavone 17 and 2,3-dihydro-6-methylginkgetin 18 exhibited potent inhibitory effects with IC(50) values of 0.75 and 0.35 microM, respectively.
Subject(s)
Amyloid Precursor Protein Secretases/antagonists & inhibitors , Biflavonoids/chemistry , Protease Inhibitors/chemistry , Amyloid Precursor Protein Secretases/metabolism , Biflavonoids/pharmacology , Protease Inhibitors/pharmacology , Structure-Activity RelationshipABSTRACT
DNA methylation at position 5 of cytosine residues plays an important role in the gene function control. The analytical method for determining the sites of 5- methylcytosine residues utilizes bisulfite treatment of genomes. Cytosines in DNA are converted into uracils by this treatment, while 5-methylcytosines remain unaltered. The bisulfite treatment followed by amplification by polymerase chain reaction and by sequencing the resulting DNA allows determination of the 5-methylcytosine sites in the original. In this chemical modification, key intermediates are those formed by addition of bisulfite across the 5,6-double bond of pyrimidine ring. Their structures were proposed in 1970 as 5,6-dihydropyrimidine 6-sulfonates, but not its 6-sulfurous acid ester, on the basis of spectral data. X-ray analysis has now been performed for a single crystal of sodium bisulfite-uracil adduct and the results showed its structure as sodium 5,6-dihydrouracil 6-sulfonate monohydrate, thus providing definite evidence for the C(6)-sulfonate structure.
Subject(s)
Sulfites/chemistry , Sulfonic Acids/chemistry , Uracil/analogs & derivatives , Crystallography, X-Ray , Models, Molecular , Uracil/chemistryABSTRACT
Cranberry, which is rich in polyphenols, including anthocyanins and proanthocyanidins, has been found to have various effects beneficial to human health, including prevention of urinary tract infections. These effects have been associated with polyphenols in the fruit. We investigated the excretion of anthocyanins in human urine after ingestion of cranberry juice. Eleven healthy volunteers consumed 200 ml of cranberry juice containing 650.8 microg total anthocyanins. Urine samples were collected within 24 h before and after consumption. Six of 12 anthocyanins identified in cranberry were quantified in human urine by HPLC coupled with electrospray ionization and tandem mass spectrometry (HPLC-ESI-MS-MS). Among these, peonidin 3-O-galactoside, the second most plentiful anthocyanin in the juice, was found most abundantly in urine within 24 h, corresponding to 41.5 nmol (56.1% of total anthocyanins). The urinary levels of anthocyanins reached a maximum between 3 and 6 h after ingestion, and the recovery of total anthocyanins in the urine over 24 h was estimated to be 5.0% of the amount consumed. This study found high absorption and excretion of cranberry anthocyanins in human urine.
Subject(s)
Anthocyanins/urine , Beverages , Vaccinium macrocarpon/chemistry , Adult , Chromatography, High Pressure Liquid , Female , Humans , Male , Spectrometry, Mass, Electrospray IonizationABSTRACT
Three new acylated flavonol glycosides, cypellogins A (1), B (2) and C (3), along with eight known phenolic compounds, were isolated from the dried leaves of Eucalyptus cypellocarpa, and their structures were elucidated using spectroscopic methods, including 2D NMR experiments and chemical evidence.
Subject(s)
Eucalyptus/chemistry , Flavonols/chemistry , Glycosides/chemistry , Plant Leaves/chemistry , Animals , Carbon Isotopes , Flavonols/isolation & purification , Glycosides/isolation & purification , Magnetic Resonance Spectroscopy/methods , Magnetic Resonance Spectroscopy/standards , Molecular Structure , Phascolarctidae , Protons , Reference StandardsSubject(s)
Antifungal Agents/chemistry , Peptides, Cyclic/chemistry , Streptomyces/chemistry , Antifungal Agents/isolation & purification , Antifungal Agents/pharmacology , Colletotrichum/drug effects , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Molecular Conformation , Peptides, Cyclic/isolation & purification , Peptides, Cyclic/pharmacology , Phyllachorales/drug effectsABSTRACT
We isolated four strains of bacteria producing antifungal antibiotics from the rhizosphere of garlic with basal rot caused by the plant pathogenic fungal strain Fusarium oxysporum. Among them, Bacillus subtilis FR-2 was found to produce new antifungal antibiotics, named bacillopeptins A, B, and C. Their structures have been determined by 1D and 2D NMR and MS experiments, and amino acid analysis coupled with chiral HPLC, to be cyclic lipopeptides each containing a long-chain beta-amino acid. Another bacterial strain, Bacillus polymyxa KT-8, was shown to produce new antifungal antibiotics named fusaricidins A, B, C, and D which are more potent than bacillopeptins in their antimicrobial activity. The structures of the fusaricidins have been elucidated similarly as bacillopeptins to be cyclic hexadepsipeptides all containing 15-guanidino-3-hydroxypentadecanoic acid as a side chain. Fusaricidins strongly inhibit the growth of various kinds of fungi and moreover surprisingly show strong inhibitory activity against Gram-positive bacteria such as Staphylococcus aureus or Micrococcus luteus.
