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1.
Prev Nutr Food Sci ; 17(3): 197-202, 2012 Sep.
Article in English | MEDLINE | ID: mdl-24471084

ABSTRACT

Normal (cv. Keumkang, KK) and waxy-type (cv. Shinmichal, SMC) whole wheat flour was substituted at 20 and 40% for white wheat flour (WF) during bread dough formulation. The flour blends were subjected to dough and baking property measurement in terms of particle size distribution, dough mixing, bread loaf volume and crumb firmness. The particle size of white wheat flour was the finest, with increasing coarseness as the level of whole wheat flour increased. Substitution of whole wheat flour decreased pasting viscosity, showing all RVA parameters were the lowest in SMC40 composite flour. Water absorption was slightly higher with 40% whole wheat flour regardless of whether the wheat was normal or waxy. An increased mixing time was observed when higher levels of KK flour were substituted, but the opposite reaction occurred when SMC flour was substituted at the same levels. Bread loaf volume was lower in breads containing a whole wheat flour substitution compared to bread containing only white wheat flour. No significant difference in bread loaf volume was observed between normal and waxy whole flour, but the bread crumb firmness was significantly lower in breads containing waxy flour. The results of these studies indicate that up to 40% whole wheat flour substitution could be considered a practical option with respect to functional qualities. Also, replacing waxy whole flour has a positive effect on bread formulation over normal whole wheat flour in terms of improving softness and glutinous texture.

2.
Mol Cell Biol ; 27(12): 4293-305, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17438128

ABSTRACT

In the developing mouse brain, the highest Bcl-X(L) expression is seen at the peak of neurogenesis, whereas the peak of Bax expression coincides with the astrogenic period. While such observations suggest an active role of the Bcl-2 family proteins in the generation of neurons and astrocytes, no definitive demonstration has been provided to date. Using combinations of gain- and loss-of-function assays in vivo and in vitro, we provide evidence for instructive roles of these proteins in neuronal and astrocytic fate specification. Specifically, in Bax knockout mice, astrocyte formation was decreased in the developing cortices. Overexpression of Bcl-X(L) and Bax in embryonic cortical precursors induced neural and astrocytic differentiation, respectively, while inhibitory RNAs led to the opposite results. Importantly, inhibition of caspase activity, dimerization, or mitochondrial localization of Bcl-X(L)/Bax proteins indicated that the differentiation effects of Bcl-X(L)/Bax are separable from their roles in cell survival and apoptosis. Lastly, we describe activation of intracellular signaling pathways and expression of basic helix-loop-helix transcriptional factors specific for the Bcl-2 protein-mediated differentiation.


Subject(s)
Cerebral Cortex/cytology , Cerebral Cortex/embryology , Gene Expression Regulation, Developmental , bcl-2-Associated X Protein/metabolism , bcl-X Protein/metabolism , Animals , Astrocytes/cytology , Astrocytes/physiology , Basic Helix-Loop-Helix Transcription Factors/metabolism , Caspase Inhibitors , Cell Differentiation , Cells, Cultured , Coated Materials, Biocompatible/metabolism , Crosses, Genetic , Dimerization , Enzyme Activation , Fibronectins/metabolism , Homozygote , Mice , Mice, Knockout , Mitochondria/metabolism , Mutagenesis, Site-Directed , Neurons/cytology , Neurons/physiology , RNA, Small Interfering/genetics , Retroviridae/genetics , Signal Transduction/physiology , Transduction, Genetic , bcl-2-Associated X Protein/chemistry , bcl-2-Associated X Protein/genetics , bcl-X Protein/chemistry , bcl-X Protein/genetics
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