NLRC3 silencing accelerates the invasion of hepatocellular carcinoma cell via IL-6/JAK2/STAT3 pathway activation.

Nucleotide-binding domain, leucine-rich repeat family with a caspase activation and recruitment domain 3 (NLRC3) participates in both immunity and cancer. The aim of this study was to determine the role of NLRC3 in human hepatocellular carcinoma (HCC) and the underlying mechanisms. We collected human liver tissues from nonalcoholic steatohepatitis (NASH), HCC, and adjacent normal tissues to characterize the pattern of NLRC3 expression by real-time quantitative polymerase chain reaction and immunohistochemistry. Then, we used the HCC cell line, HuH-7, transfected with small interfering RNA to silence the NLRC3 expression. 5-Ethynyl-2'-deoxyuridine assay, scratch assay, and transwell invasion assay were used for assessing proliferation, migration, and invasion, respectively. Flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were conducted to assess cell apoptosis. The expression of NLRC3 was reduced in human HCC tissues, compared with normal liver and nonalcoholic steatohepatitis tissues. After knocking down of NLRC3, the proliferation, migration, and invasion were increased in HuH-7 cells. And flow cytometry and TUNEL assay showed that HuH-7 cell apoptosis was suppressed after NLRC3 knockdown. As for the underlying mechanisms, knockdown of NLRC3 in HuH-7 cells was associated with the activation of Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) pathway under interleukin-6 (IL-6) stimulation. NLRC3 expression was downregulated in human HCC tissues. NLRC3 silencing in HuH-7 cells can promote the proliferation, migration, and invasion of hepatocellular carcinoma cells. JAK2/STAT3 pathway activation induced by IL-6 may be the underlying mechanism for HCC when NLRC3 expression is silenced. And the invasion of HuH-7 cells was partially suppressed by the STAT3 specific inhibitor (cryptotanshinone). Therefore, NLRC3 may play a significant role in HCC and might be a therapeutic target for the treatment of HCC.

Characteristics of phytoplankton communities and their biomass variation in a gas hydrate drilling area in the northern South China Sea.

We analyzed the data obtained from field observations on a gas hydrate drilling area in Dongsha of northern South China Sea (SCS) in middle May (before drilling) and early October (after drilling) in 2013. The variation in the phytoplankton communities and biomass as well as the impacts of environmental factors including dissolved methane was studied. Results indicated that the gas hydrate drilling area in Dongsha, SCS exhibited a typical low-nutrients low-chlorophyll a (LNLC) environment accompanied with low phytoplankton abundance. A total of 103 taxa belonging to 52 genera of 5 classes were identified, with diatoms and dinoflagellates dominating the community. Both phytoplankton abundance and chlorophyll a (Chl a) were highest at the subsurface maximum layer. The subsurface chlorophyll maximum (SCM) before and after drilling were stabilized at 75 m (0.30 ±â€¯0.06 mg/m3 and 0.51 ±â€¯0.29 mg/m3, respectively), while the subsurface maximum of abundance after drilling went deeper to 75 m (604.17 ±â€¯313.22 cells/L) from the surface (707.14 ±â€¯243.98 cells/L) before drilling. After drilling, phosphate and Chl a increased significantly, but no significant differences were observed on abundance. Dominant species of diatoms were basically constant with dinoflagellates becoming more apparent in higher occurrence and abundance, while Cyanophyta was diverse after drilling. Redundancy analysis (RDA) and Spearman's correlation analysis both indicated that temperature, pH and phosphates were major factors causing fluctuation in phytoplankton community structure, while dissolved methane had non-significant impact directly. We clearly found both abundance and Chl a increased in particular water layers (between 50 and 75 m) and at stations (DS06, DS08 and DS15) where dissolved methane concentrations were also abnormally high. This study appeared to partly coincide with the findings of natural oil seeps in the Gulf of Mexico, which assumed that the turbulence from the natural oil and gas leaking zone could raise the bottom water through the rising bubbles and bring cold nutrient rich waters to the thermocline from the deep seeps. This plume-generated upwelling could then fuel a bottom-up effect on the photosynthetic species in the upper pelagic waters within the euphotic zone.