ABSTRACT
Test anxiety and self-efficacy significantly influence the mastery of nursing skills. Facial expression recognition tools are central components to recognising these elements. This study investigated the frequent facial expressions conveyed by nursing students and examined the relationships between nursing skill mastery, test anxiety, self-efficacy, and facial expressions in a test-taking situation. Thirty-three second-year nursing students who were attending a university in a Korean metropolitan city participated. Test anxiety, self-efficacy, and facial expressions were collected while the students inserted indwelling catheters. Using Microsoft Azure software, the researchers examined the students' facial expressions. Negative facial expressions, such as anger, disgust, sadness, and surprise, were more common during the test-taking situation than the practice trial. Fear was positively correlated with anxiety. None of the facial expressions had significant relationships with self-efficacy; however, disgust was positively associated with nursing skill mastery. The facial expressions during the practice and test-taking situations were similar; however, fear and disgust may have been indicators of test anxiety and skill mastery. To create a screening tool for detecting and caring for students' emotions, further studies should explore students' facial expressions that were not evaluated in this study.
ABSTRACT
The mechanical properties of the vesicles incorporated with ectoine were studied using atomic force microscope (AFM). The vesicles were prepared with dipalmitoylphosphatidylcholine (DPPC) by changing only the ratio of the ectoine to DPPC. After the vesicles were adsorbed on the mica substrate and their morphology were characterized, the plot of an AFM tip displacement versus the tip deflection was acquired by monitoring the behavior of the tip into the vesicle. The breakthrough of the tip into the vesicle was observed to occur twice. Each breakthrough represented a penetration of the tip into the top and bottom portions of the vesicle, respectively. The force data between the pre-contact and the first breakthrough were comparable with the Hertzian model to estimate Young's modulus and the bending modulus of the vesicles. Both moduli decreased proportionally with the increase in the ratio of ectoine to lipid up to 0.5. However, above 0.5, the moduli were slightly changed with the increase. These results of the mechanical properties appear to be due to the osmotic and volumetric effect on the headgroup packing disruption.
Subject(s)
Amino Acids, Diamino , Microscopy, Atomic Force/methodsABSTRACT
The pH-sensitive liposomes were employed to amplify the detection of acetylcholine (ACh). Acetylcholinesterase (AChE) covalently immobilized on the magnetic particles and the pH-sensitive liposomes encapsulating ferricyanide were added to a cyclic voltammetry cell solution where ACh was distributed. The conversion of ACh into acetic acid seemed to induce the pH decrease that caused the reduction in the electrostatic repulsion between the head groups of weakly acidic 1,2-dipalmitoyl-sn-glycero-3-succinate. The reduction generated liposome destabilization, which released potassium ferricyanide encapsulated inside the liposomes. The effects of the ACh concentration and pH were investigated. An addition of 10 µL of more than 0.5 mg/mL ACh concentration into 5 mL of a cyclic voltammetry cell solution was necessary to observe the response. The activity of AChE was reversible with respect to the pH change between 7 and 5. The sensitivity of this detection was almost identical to comparable techniques such as enzyme-linked immunosorbent assay, field-effect transistor, fluorescence, UV spectrometry, magnetic resonance imaging, and surface plasmon resonance. Therefore, the methodology developed in this study is feasible as a portable, rapid, and sensitive method.
ABSTRACT
Oral mucosa pressure ulcers (PUs) can result in frequent pain and discomfort, and have negative effects on quality of life. We aimed to examine attitude, barriers/facilitators of oral mucosa PU prevention, the ability to differentiate oral mucosa PU, and to identify factors influencing PU prevention performance intention. This was a cross-sectional descriptive study of 112 nurses in seven tertiary hospitals and three secondary hospitals. The data collection period was from August to December 2018. For data analysis, descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient, and multiple regression were used. The mean score of attitudes toward oral mucosa PU prevention was 3.74 ± 0.39. Barriers to oral mucosa PU prevention were 5.65 ± 1.66, and facilitators were 5.35 ± 1.34. The mean correct answer rate of visual differentiation ability was 13%. The factors affecting intention to perform oral mucosa PU prevention were facilitators of oral mucosa PU prevention (ß = 0.32, p = 0.001) and attitude (ß = 0.26, p = 0.005). To increase intention to perform oral mucosa PU prevention, positive attitudes and enhanced facilitators should be encouraged. Therefore, standardized guidelines and strategies, such as educational opportunities and allocation of resources and personnel focused on oral mucosa PU prevention, need to be provided.
