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Chem Commun (Camb) ; 55(94): 14210-14213, 2019 Dec 07.
Article in English | MEDLINE | ID: mdl-31709424

ABSTRACT

Based on the full understanding of the hydrolysis patterns of duplex-specific nuclease (DSN) against the probe DNAs in DNA/microRNA heteroduplexes, a simple and generic platform for highly specific and sensitive detection of microRNAs was developed by seamlessly integrating DSN-assisted target recycling amplification and strand displacement amplification in tandem.


Subject(s)
MicroRNAs/genetics , Nucleic Acid Amplification Techniques , Cell Line, Tumor , DNA Probes/chemistry , HeLa Cells , Humans , Hydrolysis , Reverse Transcriptase Polymerase Chain Reaction
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