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1.
Appl Biochem Biotechnol ; 160(2): 428-37, 2010 Jan.
Article in English | MEDLINE | ID: mdl-18779934

ABSTRACT

A high-performance gas-liquid transmission device (HPTD) was described in this paper. To investigate the HPTD mass transfer characteristics, the overall volumetric mass transfer coefficients, K(A)(La,CO(2)) for the absorption of gaseous CO(2) and K(A)(La,O(2)) for the desorption of dissolved O(2) were determined, respectively, by titration and dissolved oxygen electrode. The mass transfer capability of carbon dioxide was compared with that of dissolved oxygen in the device, and the operating conditions were optimized to suit for the large-scale enclosed micro-algae cultivation. Based on the effectiveness evaluation of the HPTD applied in one enclosed flat plate Spirulina culture system, it was confirmed that the HPTD can satisfy the demand of the enclosed system for carbon supplement and excessive oxygen removal.


Subject(s)
Carbon Dioxide/metabolism , Culture Techniques/instrumentation , Oxygen/metabolism , Spirulina/metabolism , Absorption , Bioreactors/microbiology , Carbon Dioxide/chemistry , Equipment Design , Kinetics , Oxygen/chemistry , Spirulina/chemistry , Spirulina/growth & development
2.
Bioprocess Biosyst Eng ; 31(6): 641-5, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18369667

ABSTRACT

One simple but efficient carbon-supplying device was designed and developed, and the correlative carbon-supplying technology was described. The absorbing characterization of this device was studied. The carbon-supplying system proved to be economical for large-scale cultivation of Spirulina sp. in an outdoor raceway pond, and the gaseous carbon dioxide absorptivity was enhanced above 78%, which could reduce the production cost greatly.


Subject(s)
Biochemistry/methods , Carbon/chemistry , Eukaryota/metabolism , Industrial Microbiology/methods , Absorption , Biomass , Bioreactors , Carbon Dioxide/chemistry , Equipment Design , Gases , Hydrogen-Ion Concentration , Oxygen/chemistry , Spirulina/metabolism
3.
Bioresour Technol ; 98(4): 967-70, 2007 Mar.
Article in English | MEDLINE | ID: mdl-16624556

ABSTRACT

Biodegradation and decolorization of monosodium glutamate wastewater were carried out by using an acidophilus yeast strain of Saccharomyces cerevisiae and Coriolus versicolor. For the yeast treatment, the highest COD removal and reducing sugar removal efficiency were 76.6% and 80.2%, respectively. The color removal was only 2%. For C. versicolor treatment, the highest COD removal, color removal and reducing sugar removal efficiencies were 78.7%, 56.5% and 90.9%, respectively. The synergic treatment process, in which the yeast and C. versicolor were successively applied,exhibited great advantage over the individual process.


Subject(s)
Agaricales/metabolism , Saccharomyces cerevisiae/metabolism , Sodium Glutamate/metabolism , Water Pollutants, Chemical/metabolism , Industrial Waste
4.
Biodegradation ; 18(5): 551-7, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17146613

ABSTRACT

Degradation and decolorization of monosodium glutamate wastewater (MSGW) with Coriolus versicolor were firstly carried out. The effects of various operation parameters namely wastewater concentrations, pH, culture time and incidence of sterilization on maximum percentage of degradation and decolorization of wastewater were investigated. Studies of mycelium and enzyme for C. versicolor degradation and decolorization were estimated in this study. Ten percentage of wastewater concentration and pH = 5.0 were found to be the most suitable ones among the other experiments. The highest degradation and decolorization efficiency of wastewater was obtained at the fifth day of cultivation, which was displayed with more than 70% chemical oxygen demand removal, 83% total sugar removal and 55% color removal, respectively. Sterile operation had no remarkable effect on the degradation and decolorization efficiency for C. versicolor. Mycelium and the extra cellular fungal enzyme were both necessary for the degradation and decolorization of MSGW. C. versicolor possesses great potential and economic advantages in MSGW treatment.


Subject(s)
Biodegradation, Environmental , Sodium Glutamate/chemistry , Carbohydrates/chemistry , Hydrogen-Ion Concentration , Industrial Waste , Laccase/chemistry , Oxygen/chemistry , Polyporales/enzymology , Polyporales/metabolism , Sterilization , Time Factors , Waste Disposal, Fluid/methods , Water/chemistry , Water Pollutants, Chemical , Water Purification
5.
Biotechnol Lett ; 26(18): 1429-32, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15604776

ABSTRACT

Glucose at 3 g l(-1) markedly accelerated growth of Synechococcus sp. PCC 7002. The net photosynthesis rate was 263 micromol O2 (mg Chl a h)(-1) for mixotrophic culture and 146 micromol O2 (mg Chl a h)(-1) for photoautotrophic culture. Additing 1 g NaHCO3 l(-1) to the glucose-supplemented culture enhanced the photosynthetic rate by 18%, and the total carbon consumption rate was raised to 2.5 mg l(-1) (mg chl a h)(-1) from a previously negative value. An interaction between organic and inorganic carbon metabolism was established.


Subject(s)
Carbon Compounds, Inorganic/metabolism , Glucose/metabolism , Organic Chemicals/metabolism , Photosynthesis/physiology , Photosynthesis/radiation effects , Synechococcus/physiology , Synechococcus/radiation effects , Cell Proliferation/radiation effects , Light , Oxygen Consumption/drug effects , Oxygen Consumption/physiology
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