Wave Dispersion Behavior in Quasi-Solid State Concrete Hydration.

This paper aims to investigate wave dispersion behavior in the quasi-solid state of concrete to better understand microstructure hydration interactions. The quasi-solid state refers to the consistency of the mixture between the initial liquid-solid stage and the hardened stage, where the concrete has not yet fully solidified but still exhibits viscous behavior. The study seeks to enable a more accurate evaluation of the optimal time for the quasi-liquid product of concrete using both contact and noncontact sensors, as current set time measurement approaches based on group velocity may not provide a comprehensive understanding of the hydration phenomenon. To achieve this goal, the wave dispersion behavior of P-wave and surface wave with transducers and sensors is studied. The dispersion behavior with different concrete mixtures and the phase velocity comparison of dispersion behavior are investigated. The analytical solutions are used to validate the measured data. The laboratory test specimen with w/c = 0.5 was subjected to an impulse in a frequency range of 40 kHz to 150 kHz. The results demonstrate that the P-wave results exhibit well-fitted waveform trends with analytical solutions, showing a maximum phase velocity when the impulse frequency is at 50 kHz. The surface wave phase velocity shows distinct patterns at different scanning times, which is attributed to the effect of the microstructure on the wave dispersion behavior. This investigation delivers profound knowledge of hydration and quality control in the quasi-solid state of concrete with wave dispersion behavior, providing a new approach for determining the optimal time of the quasi-liquid product. The criteria and methods developed in this paper can be applied to optimal timing for additive manufacturing of concrete material for 3D printers by utilizing sensors.

Rapid Targeted Sequencing Using Dried Blood Spot Samples for Patients With Suspected Actionable Genetic Diseases.

Background: New genome sequencing technologies with enhanced diagnostic efficiency have emerged. Rapid and timely diagnosis of treatable rare genetic diseases can alter their medical management and clinical course. However, multiple factors, including ethical issues, must be considered. We designed a targeted sequencing platform to avoid ethical issues and reduce the turnaround time. Methods: We designed an automated sequencing platform using dried blood spot samples and a NEOseq_ACTION panel comprising 254 genes associated with Mendelian diseases having curable or manageable treatment options. Retrospective validation was performed using data from 24 genetically and biochemically confirmed patients. Prospective validation was performed using data from 111 patients with suspected actionable genetic diseases. Results: In prospective clinical validation, 13.5% patients presented with medically actionable diseases, including short- or medium-chain acyl-CoA dehydrogenase deficiencies (N=6), hyperphenylalaninemia (N=2), mucopolysaccharidosis type IVA (N=1), alpha thalassemia (N=1), 3-methylcrotonyl-CoA carboxylase 2 deficiency (N=1), propionic acidemia (N=1), glycogen storage disease, type IX(a) (N=1), congenital myasthenic syndrome (N=1), and citrullinemia, type II (N=1). Using the automated analytic pipeline, the turnaround time from blood collection to result reporting was <4 days. Conclusions: This pilot study evaluated the possibility of rapid and timely diagnosis of treatable rare genetic diseases using a panel designed by a multidisciplinary team. The automated analytic pipeline maximized the clinical utility of rapid targeted sequencing for medically actionable genes, providing a strategy for appropriate and timely treatment of rare genetic diseases.

Energy-Efficient Ultrasonic Water Level Detection System with Dual-Target Monitoring.

This study presents a developed ultrasonic water level detection (UWLD) system with an energy-efficient design and dual-target monitoring. The water level monitoring system with a non-contact sensor is one of the suitable methods since it is not directly exposed to water. In addition, a web-based monitoring system using a cloud computing platform is a well-known technique to provide real-time water level monitoring. However, the long-term stable operation of remotely communicating units is an issue for real-time water level monitoring. Therefore, this paper proposes a UWLD unit using a low-power consumption design for renewable energy harvesting (e.g., solar) by controlling the unit with dual microcontrollers (MCUs) to improve the energy efficiency of the system. In addition, dual targeting to the pavement and streamside is uniquely designed to monitor both the urban inundation and stream overflow. The real-time water level monitoring data obtained from the proposed UWLD system is analyzed with water level changing rate (WLCR) and water level index. The quantified WLCR and water level index with various sampling rates present a different sensitivity to heavy rain.