A novel coupling process with partial nitritation-anammox and short-cut sulfur autotrophic denitrification in a single reactor for the treatment of high ammonium-containing wastewater.

In this study, a novel coupling process with partial nitritation-anaerobic ammonium oxidation (anammox) (PNA) and sulfur autotrophic denitrification (SAD) was studied using an upflow biofilm reactor with mechanical vibration. At a lower dissolved oxygen (DO) concentration (0.40 ±â€¯0.20 mg L-1), ammonia could be efficiently removed from synthetic wastewater by the coupling system with a total nitrogen removal efficiency (NRE) of 98% and an influent NH4+-N concentration of 600 mg L-1. In this system, the nitrate, which was produced during the anammox reaction, could be timely reduced by the SAD reaction. Compared with the conventional PNA and SAD processes, coupling the PNA and SAD processes in a single reactor prevented nitrite accumulation in the SAD reaction and reduced the total sulfate production by 59%. The high-throughput sequencing analysis supported that the SAD bacteria (Thiobacillus) and anammox bacteria (Candidatus Kuenenia) could coexist on the elemental sulfur stone. Additionally, sulfur consumption and sulfate production were increased under a high DO concentration. The sulfate production/nitrate reduction ratio and changing profile of the substrate suggested that the short-cut SAD process mainly occurred in this coupling system. Otherwise, batch experiments also suggested that the nitrite removal rate in the anammox process was 34.5 times higher than that in the SAD process. The outcomes of these experiments revealed that most of the nitrite, as an intermediate product in the SAD reaction, served as an electron acceptor for the anammox reaction. A stoichiometric calculation of this coupling process indicated that the novel reaction scheme with a high NRE was successfully achieved. Under an ideal short-cut SAD process, almost 55% of the sulfur consumption could be reduced in this coupling system. The coupling system provides a new perspective for nitrogen removal in a single reactor and further promotes anammox and SAD performance in wastewater treatment processes.

Effects of vibration on anammox-enriched biofilm in a high-loaded upflow reactor.

An upflow biofilm reactor was operated for 211 days to investigate the effects of vibration on anammox treatment performance. With vibration, the highest nitrogen removal rates (20 kg-N·m-3·d-1) were obtained on day 180. Since the vibration could directly applied on the biofilm, it could release the dinitrogen gas accumulated in the biofilm timely and reduce the internal mass transfer resistance sharply. The specific anammox activity increased by more than 3 times with a higher vibration intensity. Meanwhile, the unique random motion caused by mechanical vibration promotes the production of extracellular proteins. Moreover, the VSS reached 20.97 g·L-1 which was 1.6 times higher than the control reactor. Such enrichment method resulted in a hard and thick anammox biofilm with a special granular morphology, and the nitrite tolerance concentration could reach 500 mg-N·L-1. Operated with an adequate vibration intensity could maintain the biofilm thickness and conducive to improve the stability of the reactor. In addition, this technique also allowed the microorganisms inside the biofilm and those on the surface to reach the same culture conditions. Base on the batch experiments, intermittent vibration caused a decrease in energy consumption from about 7.757 (kW·h)·(kg-N)-1 in group 0-Lv7(60-60) to 0.912 (kW·h)·(kg-N)-1 in group 0-Lv7(5-60). Compared to the internal recycle without vibration, the energy consumption fell by a slice over 65%. Furthermore, the high-throughput sequencing results showed that the relative abundance of Candidatus Kuenenia in reactor 1 increased from 13.2% to 43.9%.