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1.
J Thromb Haemost ; 13(11): 1980-8, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26382916

ABSTRACT

BACKGROUND: Immune tolerance induction (ITI) in patients with congenital hemophilia A is successful in up to 70%. Although there is growing understanding of predictors of response to ITI, the probability and predictors of inhibitor recurrence after successful ITI are not well understood. OBJECTIVES: To determine the association of clinical characteristics, particularly adherence to factor VIII (FVIII) prophylaxis after ITI, with inhibitor recurrence in patients with hemophilia A who were considered tolerant after ITI. METHODS: In this multicenter retrospective cohort study, 64 subjects with FVIII level < 2% who were considered successfully tolerant after ITI were analyzed to estimate the cumulative probability of inhibitor recurrence using the Kaplan-Meier method. The association of clinical characteristics with inhibitor recurrence was assessed using logistic regression. RESULTS: A recurrent inhibitor titer ≥ 0.6 BU mL(-1) occurred at least once in 19 (29.7%) and more than once in 12 (18.8%). The probability of any recurrent inhibitor at 1 and 5 years was 12.8% and 32.5%, respectively. Having a recurrent inhibitor was associated with having received immune modulation during ITI (odds ratio [OR] 3.8, 95% confidence interval [CI] 1.2-22.4) and FVIII recovery of < 85% at the end of ITI (OR 2.6, 95% CI 1.3-5.9) but was not associated with adherence to post-ITI prophylactic FVIII infusion (OR 0.5, 95% CI 0.06-4.3). CONCLUSIONS: The use of immune modulation therapy during ITI and lower FVIII recovery at the end of ITI appear to be associated with an increased risk of inhibitor recurrence after successful ITI. Adherence to post-ITI prophylactic FVIII infusions is not a major determinant of recurrence.


Subject(s)
Factor VIII/immunology , Hemophilia A/immunology , Immunosuppression Therapy , Isoantibodies/biosynthesis , Child , Child, Preschool , Factor VIII/administration & dosage , Factor VIII/therapeutic use , Female , Hemophilia A/drug therapy , Humans , Immunosuppressive Agents/therapeutic use , Infant , Isoantibodies/blood , Isoantibodies/immunology , Kaplan-Meier Estimate , Logistic Models , Male , Medication Adherence , Models, Immunological , Plasmapheresis , Propensity Score , Recombinant Proteins/immunology , Recombinant Proteins/therapeutic use , Recurrence , Retrospective Studies , Time Factors
2.
Thromb Haemost ; 113(2): 373-84, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25354705

ABSTRACT

The C-terminal C domains of activated coagulation factor VIII (FVIIIa) are essential to membrane binding of this crucial coagulation cofactor protein. To provide an overall membrane binding mechanism for FVIII, we performed simulations of membrane binding through coarse-grained molecular dynamics simulations of the C1 and C2 domain, and the combined C-domains (C1+C2). We found that the C1 and C2 domain have different membrane binding properties. The C1 domain uses hydrophobic spikes 3 and 4, of its total of four spikes, as major loops to bind the membrane, whereas all four of its hydrophobic loops of the C2 domain appear essential for membrane binding. Interestingly, in the C1+C2 system, we observed cooperative binding of the C1 and C2 domains such that all four C2 domain spikes bound first, after which all four loops of the C1 domain inserted into the membrane, while the net binding energy was higher than that of the sum of the isolated C domains. Several residues, mutations of which are known to cause haemophilia A, were identified as key residues for membrane binding. In addition to these known residues, we identified residues from the C1 and C2 domains, which are involved in the membrane binding process, that have not been reported before as a cause for haemophilia A, but which contribute to overall membrane binding and which are likely candidates for novel causative missense mutations in haemophilia A.


Subject(s)
Factor VIII/chemistry , Blood Coagulation , Cell Membrane/metabolism , Hemophilia A/genetics , Hemophilia A/metabolism , Humans , Hydrophobic and Hydrophilic Interactions , Imaging, Three-Dimensional , Molecular Dynamics Simulation , Mutation , Phosphatidylcholines/chemistry , Protein Binding , Protein Structure, Secondary , Protein Structure, Tertiary
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