Simultaneous colorimetric and surface-enhanced Raman scattering detection of melamine from milk.

We present a dual-mode readout sensing mechanism that can effectively distinguish true and false-positive signals of melamine in milk by combining colorimetric analysis and surface-enhanced Raman scattering (SERS) spectroscopy. The colorimetry analysis takes advantage of color change of plasmonic nanoparticles upon the presence of melamine. We discovered that Ag colloids with 20 nm diameter was suitable for both colorimetric and SERS methods. However, the colorimetric method may present false-positive signals with the presence of interfering compounds. SERS spectroscopy can overcome this limitation and directly obtain signature spectra from the same plasmonic NPs used for the colorimetric assay without any modification. Melamine/s-triazine can be reliably differentiated by probing the SERS spectra based on surface-selection rules. The limit of detection of sensing melamine from milk by this method could reached to 0.05 ppm. Therefore, the combination of colorimetric and SERS method not only allows for rapid preliminary screening of melamine by naked eyes, but also greatly reduces false-positive signals by surface selection rules in SERS.

Plasmonic cellulose textile fiber from waste paper for BPA sensing by SERS.

Flexible plasmonic Surface-enhanced Raman scattering (SERS) substrates were fabricated using cellulose textile fibers, in which the textile fibers were recycled from waste paper in an eco-friendly way. The Glycidyltrimethylammonium chloride (GTAC) with positive charges was grafted onto the surface of the cellulose textile fibers through cationization. Plasmonic silver nanoparticles (Ag NPs) with negative charges were decorated onto the cellulose textile fibers via electrostatic interactions. After cationization, the variation range of the diameter of the cellulose textile fibers was significantly increased because part of the cellulose was dissolved under alkaline condition, leading to more 'hot spots' for SERS during the shrinking process. The cellulose textile fiber-Ag NPs nanocomposite was employed for monitoring bisphenol A (BPA) in water and soft drink by SERS and the sensitivity of BPA detection achieved 50 ppb. The recovery values of BPA in soda water samples were from 96% to 105%. These results illustrate that the cellulose textile fiber-Ag NPs nanocomposite can be used as flexible, high sensitivity SERS substrates for detecting harmful ingredients in food or environment.

Quantum Dot Fullerene-Based Molecular Beacon Nanosensors for Rapid, Highly Sensitive Nucleic Acid Detection.

Spherical fullerene (C60) can quench the fluorescence of a quantum dot (QD) through energy-transfer and charge-transfer processes, with the quenching efficiency regulated by the number of proximate C60 on each QD. With the quenching property and its small size compared with other nanoparticle-based quenchers, it is advantageous to group a QD reporter and multiple C60-labeled oligonucleotide probes to construct a molecular beacon (MB) probe for sensitive, robust nucleic acid detection. We demonstrated a rapid, high-sensitivity DNA detection method using the nanosensors composed of QD-C60-based MBs carried by magnetic nanoparticles. The assay was accelerated by first dispersing the nanosensors in analytes for highly efficient DNA capture resulting from short-distance three-dimensional diffusion of targets to the sensor surface and then concentrating the nanosensors to a substrate by magnetic force to amplify the fluorescence signal for target quantification. The enhanced mass transport enabled a rapid detection (<10 min) with a small sample volume (1-10 µL). The high signal-to-noise ratio produced by the QD-C60 pairs and magnetic concentration yielded a detection limit of 100 fM (∼106 target DNA copies for a 10 µL analyte). The rapid, sensitive, label-free detection method will benefit the applications in point-of-care molecular diagnostic technologies.

Metal assisted focused-ion beam nanopatterning.

Focused-ion beam milling is a versatile technique for maskless nanofabrication. However, the nonuniform ion beam profile and material redeposition tend to disfigure the surface morphology near the milling areas and degrade the fidelity of nanoscale pattern transfer, limiting the applicability of the technique. The ion-beam induced damage can deteriorate the performance of photonic devices and hinders the precision of template fabrication for nanoimprint lithography. To solve the issue, we present a metal assisted focused-ion beam (MAFIB) process in which a removable sacrificial aluminum layer is utilized to protect the working material. The new technique ensures smooth surfaces and fine milling edges; in addition, it permits direct formation of v-shaped grooves with tunable angles on dielectric substrates or metal films, silver for instance, which are rarely achieved by using traditional nanolithography followed by anisotropic etching processes. MAFIB was successfully demonstrated to directly create nanopatterns on different types of substrates with high fidelity and reproducibility. The technique provides the capability and flexibility necessary to fabricate nanophotonic devices and nanoimprint templates.

Subwavelength focusing of terahertz waves in silicon hyperbolic metamaterials.

We theoretically demonstrate the subwavelength focusing of terahertz (THz) waves in a hyperbolic metamaterial (HMM) based on a two-dimensional subwavelength silicon pillar array microstructure. The silicon microstructure with a doping concentration of at least 1017 cm-3 offers a hyperbolic dispersion at terahertz frequency range and promises the focusing of terahertz Gaussian beams. The results agree with the simulation based on effective medium theory. The focusing effect can be controlled by the doping concentration, which determines the real part of the out-of-plane permittivity and, therefore, the refraction angles in HMM. The focusing property in the HMM structure allows the propagation of terahertz wave through a subwavelength aperture. The silicon-based HMM structure can be realized using microfabrication technologies and has the potential to advance terahertz imaging with subwavelength resolution.

Quasi-Optical Terahertz Microfluidic Devices for Chemical Sensing and Imaging.

We first review the development of a frequency domain quasi-optical terahertz (THz) chemical sensing and imaging platform consisting of a quartz-based microfluidic subsystem in our previous work. We then report the application of this platform to sensing and characterizing of several selected liquid chemical samples from 570⁻630 GHz. THz sensing of chemical mixtures including isopropylalcohol-water (IPA-H2O) mixtures and acetonitrile-water (ACN-H2O) mixtures have been successfully demonstrated and the results have shown completely different hydrogen bond dynamics detected in different mixture systems. In addition, the developed platform has been applied to study molecule diffusion at the interface between adjacent liquids in the multi-stream laminar flow inside the microfluidic subsystem. The reported THz microfluidic platform promises real-time and label-free chemical/biological sensing and imaging with extremely broad bandwidth, high spectral resolution, and high spatial resolution.

Photo-induced spatial modulation of THz waves: opportunities and limitations.

Programmable conductive patterns created by photoexcitation of semiconductor substrates using digital light processing (DLP) provides a versatile approach for spatial and temporal modulation of THz waves. The reconfigurable nature of the technology has great potential in implementing several promising THz applications, such as THz beam steering, THz imaging or THz remote sensing, in a simple, cost-effective manner. In this paper, we provide physical insight about how the semiconducting materials, substrate dimension, optical illumination wavelength and illumination size impact the performance of THz modulation, including modulation depth, modulation speed and spatial resolution. The analysis establishes design guidelines for the development of photo-induced THz modulation technology. Evolved from the theoretical analysis, a new mesa array technology composed by a matrix of sub-THz wavelength structures is introduced to maximize both spatial resolution and modulation depth for THz modulation with low-power photoexcitation by prohibiting the lateral diffusion of photogenerated carriers.