ABSTRACT
The start of colo-rectal laparoscopic surgery, after an initial period of enthusiasm tempered by predictable technical difficulties, was finally slowed down by the risk of port site metastases. However, this kind of surgery continued to be used for benign colonic pathology, allowing the achievement of surgical skill. Some well-trained teams reported comparable loco-regional relapse rate and 5-years survival in both laparoscopic and conventional trials. The some good results concern also mortality and morbidity in both kind of trials. Based on 613 personal cases, the authors reveal this surgery's peculiarities, justifying a predictable development.
Subject(s)
Colonic Diseases/surgery , Laparoscopy/methods , Adolescent , Adult , Aged , Aged, 80 and over , Colorectal Neoplasms/surgery , Diverticulitis, Colonic/surgery , Female , Humans , Male , Middle Aged , Rectal Diseases/surgery , Survival AnalysisABSTRACT
OBJECTIVE: To investigate the relationship between number and location of allelic imbalances (AI) and local tumor progression according to Astler-Coller classification. SUMMARY BACKGROUND DATA: Spontaneous errors in DNA replication (i.e., allelic imbalance or microsatellite instability) have been suggested to play an important role in carcinomatous transformation as reflecting alterations of gene function. METHODS: One hundred two consecutive patients with colorectal carcinoma undergoing surgical resection were included in this study. Patients were distributed according to the Astler-Coller classification as stages A (n = 7), B1 (n = 15), B2 (n = 24), C (n = 31), and D (n = 25). Fluorescent polymerase chain reaction was performed on frozen tumor, normal colon mucosa, and blood DNA at 35 microsatellite markers. Allelic imbalance frequency was compared with tumor staging. RESULTS: The percentage of AI was significantly higher in stage D than in A/B1 and B2. In addition, the percentage of AI was significantly higher in 10 synchronous colorectal liver metastases than in stage A/B1 and B2 tumors. However, the allelotyping revealed a subgroup of A/B1 tumors with a high AI frequency. Statistical analysis showed that the presence of AI at microsatellites D1S305, D2S138, D3S1282, D17S790, and D22S928 presented a significantly positive correlation with stages. CONCLUSION: The frequency of AI significantly correlates with tumor progression of colorectal cancer. Primary tumors with synchronous colorectal liver metastases showed a higher percentage of AI, suggesting that a frequency of AI greater than 35% with this selection of markers indicates a high risk of local progression and of development of metastases.
Subject(s)
Allelic Imbalance , Colorectal Neoplasms/genetics , Liver Neoplasms/secondary , Adult , Aged , Aged, 80 and over , Colorectal Neoplasms/pathology , Disease Progression , Female , Gene Frequency , Humans , Male , Microsatellite Repeats/genetics , Middle Aged , Polymerase Chain Reaction , PrognosisABSTRACT
PURPOSE OF THE STUDY: To quantify the modifications occurring on the femoral side, after Total Knee Arthroplasty, we describe a method of measurements in the sagittal plane on standardized X-rays. MATERIAL AND METHODS: We report the clinical and radiologic results of a prospective study of 80 cases of Total Knee Arthroplasty performed with the same prosthesis. We eliminated twenty cases with incorrect X-rays. Measurements were done on 60 preoperative X-rays and 60 postoperative X-rays. The aim of the measure was to quantify the modifications induced by the prosthesis on the trochlear groove and on the tibio-femoral joint line in full extension and in flexion, to eliminate individual femoral diameter was used as a reference in order to cancel variations in X-rays magnification. We first validated the two perpendicular reference axis on which we based all the measurements. RESULTS: No significative difference was found on the position of the tibio-femoral joint line in flexion. The tibio-femoral joint line in extension was significantly moved distally. The prosthesis was placed too distally, therefore we changed the operative procedure. No significative difference was found on the position of the anterior trochlear groove. DISCUSSION: With this method, the anatomic variations of the femoral profiles can be quantified, therefore, further prosthesis shapes can be improved. Radiological follow-up of knee prosthesis and comparison of the influence of different prosthesis can be made by these measures.