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Indian J Pathol Microbiol ; 63(2): 210-213, 2020.
Article in English | MEDLINE | ID: mdl-32317517

ABSTRACT

OBJECTIVES: The aim of this study was to assess the utility of Xpert assay, Ziehl-Neelsen (ZN) staining, and Mycobacteria Growth Indicator Tube (MGIT™) culture for diagnosis of pediatric pulmonary tuberculosis from gastric aspirate (GA) samples and to compare Xpert assay and ZN staining with MGIT rapid liquid culture. MATERIALS AND METHODS: GA samples from 210 nonexpectorating children, aged between 6 months to 12 years, presenting to the pediatric out-patient department (OPD) with clinical suspicion of tuberculosis (TB) were collected. The samples were tested by GeneXpert, ZN staining, and MGIT liquid culture. RESULTS: GeneXpert is a more sensitive method for rapid and early diagnosis of pediatric TB when compared with microscopy.


Subject(s)
Gastrointestinal Contents/microbiology , Mycobacterium tuberculosis/isolation & purification , Tuberculosis, Pulmonary/diagnosis , Child , Child, Preschool , Colony Count, Microbial , Female , Humans , Infant , Male , Nucleic Acid Amplification Techniques , Sensitivity and Specificity , Sputum/microbiology , Tertiary Care Centers
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