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J Chromatogr Sci ; 55(7): 750-756, 2017 Aug 01.
Article in English | MEDLINE | ID: mdl-28383683

ABSTRACT

A rapid and sensitive assay method has been developed and validated using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode for the estimation of SF0034 in mice plasma. The assay procedure involves a simple protein precipitation of SF0034 and tolbutamide (internal standard, IS) from mice plasma. Chromatographic separation was performed on an Atlantis dC18 column using an isocratic mobile phase comprising 0.2% formic acid:acetonitrile (10:90, v/v) at a flow rate of 0.60 mL/min. The total run time was 2.5 min. For mass spectrometric detection, the multiple reaction monitoring was used and ion transitions monitored were m/z 322 → 248 for SF0034 and 271 → 155 for IS. Method validation was performed as per regulatory guidelines and the results met the acceptance criteria. A calibration curve was constructed in the range of 2.08-2,078 ng/mL. The intra- and inter-day precision was in the range of 1.06-14.4% and 7.16-11.7%, respectively. This novel method has been applied to a pharmacokinetic study in mice.


Subject(s)
Carbamates/blood , Chromatography, Liquid/methods , Phenylenediamines/blood , Tandem Mass Spectrometry/methods , Animals , Carbamates/chemistry , Carbamates/pharmacokinetics , Linear Models , Male , Mice , Mice, Inbred BALB C , Phenylenediamines/chemistry , Phenylenediamines/pharmacokinetics , Reproducibility of Results , Sensitivity and Specificity
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