1.
Mol Cell Biochem
; 219(1-2): 39-44, 2001 Mar.
Article
in English
| MEDLINE
| ID: mdl-11354251
ABSTRACT
Three acidic phospholipases A2 from Indian cobra (Naja naja naja) venom inhibited platelet aggregation in platelet rich plasma induced separately by ADP, collagen and epinephrine with different potencies. The order of inhibition was epinephrine > collagen > ADP. They did not inhibit platelet aggregation induced by arachidonic acid (10 microM). The inhibition was dependent on concentration of the protein and the time of incubation of the phospholipases A2 with platelet rich plasma. Parabromophenacyl bromide modified PLA2 enzymes lost their enzymatic activity as well as platelet aggregation inhibition activity suggesting the involvement of catalytic function in platelet aggregation inhibitory activity.
Subject(s)
Acetophenones/pharmacology , Catalysis , Elapid Venoms/enzymology , Epinephrine/pharmacology , Phospholipases A/metabolism , Platelet Aggregation Inhibitors/pharmacology , Adenosine Diphosphate/pharmacology , Adrenergic Agonists/pharmacology , Animals , Blood Platelets/drug effects , Blood Platelets/metabolism , Collagen/pharmacology , Dose-Response Relationship, Drug , Elapid Venoms/chemistry , Elapid Venoms/isolation & purification , Enzyme Inhibitors/pharmacology , Humans , In Vitro Techniques , Isoenzymes/isolation & purification , Isoenzymes/metabolism , Phospholipases A/isolation & purification , Phospholipases A2 , Time Factors
2.
Biochem J
; 128(1): 59P, 1972 Jun.
Article
in English
| MEDLINE
| ID: mdl-5085645