ABSTRACT
It is proposed to combine the traditional method of microscopic assessment of the results of absorption-elution reaction and the gel-filtration technique with the use of the ScanGel immunohematology system (Bio-Rad Laboratories, USA) in blood grouping tests. The study was designed to select monoclonal antibodies suitable for the purpose and optimize conditions of gel filtration. It is concluded that the combined method allows for rapid differential grouping of the large number of blood spots, promotes objectivization of assay results, and lightens the work of the operator carrying out serial analyses.
Subject(s)
ABO Blood-Group System , Blood Grouping and Crossmatching/methods , Blood Stains , Hemagglutination Tests/methods , ABO Blood-Group System/immunology , Antibodies, Monoclonal , Forensic Medicine/methods , Humans , Reproducibility of ResultsABSTRACT
A method is proposed for ABO grouping of human hair microfragments 1.5-2.0 cm in length. The method is a modification of the absorption-elution reaction (AER) for monoclonal antibodies (MCA). MCA manufactured by Gematolog Ltd. (Russia) as well as Bioclone anti-A and anti-B reagents (Ortho-Clinical Diagnostics, USA) proved suitable for hair typing. A series of procedures for sample preparation and treatment in the reaction, such as the heating of a hair stem on a boiling water bath and the addition of a non-ionic detergent (Tween 20) to the working solution, were used to prevent non-specific results of the analysis. It is emphasized that each analysis must be made in duplicate.
